Tumor+cDNA+Library+Plasmids+from+Miniprep+1-8-12

This is a miniprep from a pooled culture. Many different 200 uL wells (2499 wells) containing 78.9 colonies per well on average were grown overnight (some of these may have been lost). The original transformation efficiency of these cultures was 9.8*10^5 cfu/ug. 10 uL from each well was pooled into one culture, and this culture was miniprepped.

Altogether there should be about 200*946.8 = 189,360 cfu = 1.9*10^5 cfu