SfiI+Site

Restriction Site

GGCCNNNNNGGCC



Actual Sequence in PCANTAB plasmid ggcccagccggcc
 * Unique part of Sequence: cagcc

Actual Sequence in scFv MHV_BACK_SfiI primer
 * GGCCCAGCCGGCC
 * Unique part
 * CAGCC

Actual Sequence in pTriplEx2
 * SfiIA Site
 * GGCCATTACGGCC
 * Unique part: ATTAC
 * Note that there was a discrepancy between the SMART cDNA library and the pTriplEx2 vector sequence information. After contacting Clontech, they responded (12-6-11) and informed me that they think the user manual has the correct sequence. So "ATTAC" is the correct sequence.
 * SfiIB Site
 * GGCCATTACGGCC
 * Unique part: GCCTC