Instructions+for+constructing+Figure+2b+heatmap+10-28-12

Obtain naive gpr files

S:\Research\Cancer_Eradication\Users\kwhittem\kwhittem\kwhittem\Records in CIM Folder\Dated Records\12-21-09 Unpurified Rabbit SMC1 on array\12-21-09 array files Obtain smc1 gpr files

S:\Research\Cancer_Eradication\Users\kwhittem\kwhittem\Raw Data Often Originally on Research Drive\3-18-10\results

Put these gpr files in a new folder and drag into Genespring. Create a new experiment without any normalizations. Alternatively, use the Genespring experiment already created.

File->Open Genome or Array->data->Alta 10K

Select the sorted random peptide list

Gene Lists->Kurt->SMC1 temp Analysis with Phil on 5-6-10->108 peptides sorted by scores from raw values Select the experiment: Experiments->Kurt->031810 Blocked by SMC1 20 Steps 2->Default interpretation

Then make the heatmap by going to tools->clustering->condition tree and use all of the default paramaters

Change the lower color to green and the upper color to red Change the colorbar range. High 30000 Normal 3000 Low 0