pComb3XSS+Vector

Size: 4973 Sequence: http://www.scripps.edu/mb/barbas/content/pcomb_images/pcomb_images_files/pComb_Text_Files/pComb3XSS.txt Original Map from Scripps: http://www.scripps.edu/mb/barbas/content/pcomb_images/pcomb_images_files/pComb_Maps/pComb3X_Maps.pdf

NotI = GCGGCCGC SpeI = ACTAGT SfiI = GGCCNNNNNGGCC

NotI = 2570bp SpeI = 1940bp SfiI = 274bp, and 1946bp (GGCCCAGGCGGCC, GGCCAGGCCGGCC)

Fragment sizes when cutting with SpeI and NotI 630bp 4343bp Sequence of Fragment Cut by SpeI and 060612

some potential annotations > amp 3174-4034 > f1 ori 2720-3026 > pBR322 ori 4190-4809 > Light Chain Stuffer from SacI to XbaI 293-1473 > Heavy Chain Stuffer from XhoI to SpeI 1580-1940 > geneIII from amber stop codon to NheI 2016-2552 > SacI 293 (GAGCTC) > XbaI 1473 > XhoI 1580 > SpeI (ACTAGT) 1940 > NheI (GCTAGC) 2552 > amber stop codon (TAG) 2016 > SfiI_1 274 (GGCCCAGGCGGCC) > SfiI_2 1946 (GGCCAGGCCGGCC) > NotI 2570 > Plan for modifying pComb3XSS vector 061312
 * lac prom 145-174

Note that a new vector was made from this vector: pComb3XSSv2 pComb3XSSv3

Request of pComb3XSS vector e-mails regarding request of pComb3XSS vector 3-25-12 pComb3xSS ships June 4th 2012! Received vector from Scripps 6-5-12 pComb3XSS 10 uL of 10 ng/uL at -20 C