PCR+by+starting+with+a+small+amount+of+primer+and+later+adding+more+primer+and+polymerase+portal+4-11-13

This was a technique first suggested by Andrey which seems to work quite well. Using a small amount of primer to begin with allows the primers to find their exact sequence. Then they can amplify the sequence. Then when there are several of the correct sequences to amplify, more primer can be added. Now that there is more correct product to start from it is more likely that these additional primers will not just bind none specifically unless too much primer is added of course.

What's a typical final primer concentration? About 0.2-1 uM according to http://www.scientistsolutions.com/t8484-whats+a+good,+typical+final+primer+conc_.html

One of the first attempts around 8-25-10 Another attempt around 4-4-13

With PrimeSTAR Max perhaps a reasonable procedure would be to add 1 uL of 2 uM each primer (0.04 uM final concentration each) and do 25 cycles. Then add 0.5-1 uL 10 uM each primer (0.14 uM or 0.24 uM respectively total concentration each primer at this point), 5 uL H20, 5 uL PrimeSTAR Max Premix, and perform 5 more cycles, save an aliquot (10 uL), do 5 more cycles, save an aliquot, and do this until there are no longer any more aliquots to save.