1st+Ova+Spleen+Experiment

Pictures of Targets and Effectors on 4th day of induction incubation (pictures taken on 10-24-11)



Pictures with edits by Vanessa

Ova Immunization ELISA 10-24-11

Results of flow cytometry: 10-26-11









"C:\kurt\storage\CIM Research Folder\DR\2013\1-24-13\data_download\2015\Raw data.zip"

Notes: We were not able to detect any killing. There is one major possible reason for this. The first reason was that we were not able to detect the killing of the NIH 3T3 cells in the positive control with camptothecin whereas we were able to detect killing when using Jurkat cells.

Another potential problem is that the NIH 3T3 cells we used may not have the correct MHC haplotype. Here is a message from Shen about this:

I found NIH-3T3 MHC haplotype is H-2q, while BALB/c is H-2d.

See following link: @http://www.bio.net/bionet/mm/immuno/1999-March/013999.html

More information about MHC haplotypes can be found here

Ova Immunization ELISA 10-24-11