Ordering+primers+to+verify+sequence+10-25-12

Ordering primers to verify sequence 10-25-12

There were some odd things around where the NotI site was removed in the pComb MN site, but this was towards the end of the sequence. I will order some new primers to determine what this sequence is exactly.

see Plan for modifying pComb3XSS vector 061312

potential primer at 2362 of pcomb MN plasmid MNVF (modified notI verification forward) 5' CTTCCCTCCCTCAATCGG 3' Oligo Master Tm: 52.5 C

primer located at 2381 ("C:\kurt\storage\CIM Research Folder\DR\2012\7-26-12\pcomb sequence analysis\generating new pcomb sequence 7-28-12.SPF")

Reverse primer MNVR (modified notI verification reverse) CTCCAACGTCAAAGGGCG appropriate reverse complement sequence

Oligo Master Tm: 52.5 C

primer located at 2776 of pcomb MN

10-31-12 received pcomb validation primers