Flow+Cytometry+Cytotoxicity+Assay

We can keep notes about how we want to do the flow cytometry cytotoxicity assay here. These notes can be linked to new pages as well of course.

Overall diagram of flow cytometry

Flow Cytometry CTL Protocol Alternative CTL Assays to CRA Flow Cytometer Biodesign Equipment Experiments Map of Experiments for CTL Flow Cytometry Assay Discussions Miscellaneous Information

Here is the wikepedia page on Flow Cytometry that I read to get a better idea of what it is, and I made some notes on it too. "C:\kurt\storage\CIM Research Folder\DR\2013\2-2-13\data_download\1050\flow cytometry_wikipedia with comments.pdf"

I found this website that has a flow cytometry "glossary." Just thought I'd add it here in case it's helpful at some point. http://cyto.mednet.ucla.edu/Protocols/flow.htm

Here is the BD Biosciences Training Material @http://www.bdbiosciences.com/support/training/itf_launch.jsp

A Survey of Flow Cytometry Data Analysis Methods (annotated) "C:\kurt\storage\CIM Research Folder\DR\2013\2-2-13\data_download\1050\A survey of Flow Cytometry Data Analysis Methods_annotated VB.pdf"

The paper didn't help too much understand how Flow Cytometry works but it was interesting to read about the methods to interpret the data because it seems there are several different ways to do so for different purposes. It's not just a straight-shot measurement. It might be a good resource for when we get to data analysis.

Here's the link to the flow cytometry videos from Tiana. @http://www.bdbiosciences.com/support/training/itf_launch.jsp

I made this little diagram of how the FACS flow cytometry works, just to get a better idea of it, so I thought I'd share. (Don't make fun of my drawing skillz please :) ). I figured this is the very simplistic way of looking at it, and I'm sure we'll change our "diagrams" as we know more and develop more of a protocol.



Questions What level of detection do we have to reach with our test to prove that it is comparable with the chromium release assay?