Tumor+vs+normal+on+2-17-12_16E3+spot

I will apply sera to arrays created from the 2-17-12_16E3 tumor spot. I will use 1 nM secondary. I won't use a direct labelled secondary so that the streptavidin dye can bind to the biotinylated peptide landing lights.

actual experiment on 7-3-12

4 tumor sera slides 4 naive sera slides

Array Printing Scheme Tumor Library Nitrocellulose Print 7-1-12

Mara aligned the slides and performed some of the statistical analysis. S:\Research\Cancer_Eradication\Discovering tumor specific antigens\Tumor cDNA Library\Application of sera onto array\7-4-12 naive_tumor scan I copied some of this to this folder C:\kurt\storage\CIM Research Folder\DR\2012\7-5-12\Array Analysis