Western+Blot+check+of+SMC1fs+production+in+cell+lysate+with+varied+conditions

The purpose of this experiment was to make sure that bacteria produced the expected protein (SMC1fs) when the bacteria were induced in a 96 well plate format. The bacteria were subjected to varying conditions to determine what the best conditions were.

Expected GST-SMC1fs size: 29.2 kD Dye Used: AF647 Excitation wavelength: 633 nm Emission wavelength: 670 nm Ladder used: Precision Plus Dual Color Standard

Samples all at 30 C in this image





Note: I think this membrane with the 6hr samples does not look as good because the sponge used to dry it fell onto the counter beforehand. The bands are still visible and quite strong though.

Primary Sera Source: Purified Rabbit h-SMC1fs 0.73 mg/mL 8/7/07 (diluted 1:1,000) Secondary Sera Source: Goat anti rabbit IgG h+l 1 mg/mL (diluted 1:10,000) Tertiary Source: AF647 2 mg/mL (diluted 1:10,000)

Mouse sera was also tested These mice were only genetically immunized



Note: The TEV-thioredoxin-His tag appears to bind the antibody quite well too, or there was too much lysozyme in the negative control.

Supporting Files ->