Diversity+of+HIV-1+Vpr+interactions+involves+usage+of+the+WXXF+motif+of+host+cell+proteins.

Diversity of HIV-1 Vpr Interactions Involves Usage of the WXXF Motif of Host Cell Proteins

BouHamdan, M., Xue, Y., Baudat, Y., Hu, B., Sire, J., Pomerantz, R.J., Duan, L.-X., 1998. Diversity of HIV-1 Vpr interactions involves usage of the WXXF motif of host cell proteins. Journal of Biological Chemistry 273, 8009-8016.

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Diversity of HIV-1 Vpr Interactions Involves Usage of the WXXF Motif of Host Cell Proteins notes 11-4-12

(Received for publication, October 9, 1997, and in revised form, December 9, 1997) Mohamad BouHamdan‡§, YanNing Xue‡§, Yves Baudat¶, Baocheng Hu‡, Josephine Sire¶, Roger J. Pomerantz‡, and Ling-Xun Duan‡i From the ‡The Dorrance H. Hamilton Laboratories, Center for Human Virology, Division of Infectious Diseases, Department of Medicine, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania 19107 and ¶INSERM U372, Pathogenie des infections a lentivirus, BP 178, 13276 Marseille-Cedex 9, France

long term goal would be to make an anti-viral agent to interfere with HIV-1 replication

q Applying this target protein docking model for further molecular analyses is now possible due to the development of the phage display system

Vpr protein very important for HIV virus

q We demonstrate that most of the peptides that bind to Vpr contain a common motif, WXXF.

q A phage-display peptide library kit (New England Biolabs, Beverly, MA)

transfection protocol

? I don't know the purpose of the CAT assays. Probably will become clear later.

His-deficient medium. ? don't know the significance of His deficient medium

q These results clearly indicate that the amino acids Trp (222) and Phe (225) of UDG, corresponding to the WXXF motif of UDG, are necessary for binding to Vpr.

they fused WXXF to the CAT gene CAT is a foreign protein to HIV-1

Growth in the absence of histidine is an indication of the interaction between hybrid proteins. (in a yeast two hybrid system I guess)

UDG contains WXXF

q This study illustrates a potential new strategy in the targeting of anti-viral agents into virions that may permit a feasible avenue to interfere with HIV-1 replication in vivo.