Cell+Lysate+Plate+9-21-11

for formatted version see OneNote Document: "F:\kurt\storage\CIM Research Folder\DR\2013\6-16-13\wiki_download\Cell Lysate Plate 9-21-11.one" Cell Lysate Plate 9-21-11 Wednesday, September 21, 2011 7:10 PM Denatured proteins: 1% SDS 1 hr 60 C (50 uL original sample) Plate Layout EDTA was added to samples (final concentration: 1 mM) to prevent bacterial growth. GST was obtained from Shen's shelf: GST protein from pGEX2T in PBS-2 1/28/11 SMC1 protein was obtained from Shen's shelf hSMC1-27mer 1 mg/mL 6/28/11 I diluted this protein 5 fold (40 uL into 200 uL 1X PBS) for 0.2 mg/mL Nanodrop Concentrations:
 * || 1 || 2 || 3 || 4 || 5 || 6 || 7 || 8 || 9 || 10 || 11 || 12 || 13 || 14 || 15 || 16 || <span style="color: #000000; display: block; font-family: Calibri; font-size: 14.6667px; text-align: right;">17 || <span style="color: #000000; display: block; font-family: Calibri; font-size: 14.6667px; text-align: right;">18 || <span style="color: #000000; display: block; font-family: Calibri; font-size: 14.6667px; text-align: right;">19 || <span style="color: #000000; display: block; font-family: Calibri; font-size: 14.6667px; text-align: right;">20 ||
 * A (non-denatured) || 1X || 0.5X || 0.1X || 0.01X || 0.001X || 0.0001X || <span style="color: #000000; display: block; font-family: Calibri; font-size: 14.6667px; text-align: right;">0 || GST || GST-SMC1fs || Old 1X from 8-10-11 || 1X || 0.5X || 0.1X || 0.01X || 0.001X || 0.0001X || <span style="color: #000000; display: block; font-family: Calibri; font-size: 14.6667px; text-align: right;">0 || GST || GST-SMC1fs || Old 1X from 8-10-11 ||
 * B ||  ||   ||   ||   ||   ||   ||   ||   ||   ||   ||   ||   ||   ||   ||   ||   ||   ||   ||   ||   ||
 * C (denatured) || 1X || 0.5X || 0.1X || 0.01X || 0.001X || 0.0001X || <span style="color: #000000; display: block; font-family: Calibri; font-size: 14.6667px; text-align: right;">0 || GST || GST-SMC1fs || Old 1X from 8-10-11 || 1X || 0.5X || 0.1X || 0.01X || 0.001X || 0.0001X || <span style="color: #000000; display: block; font-family: Calibri; font-size: 14.6667px; text-align: right;">0 || GST || GST-SMC1fs || Old 1X from 8-10-11 ||
 * Sample || Concentration (ng/mL) || 260/280 ||
 * pGEX-SMC1fs Induced || 11.54 || 0.82 ||
 * pGEX-SMC1fs Not Induced || 13.46 || 0.73 ||
 * pET32 TEV || 8.58 || 0.55 ||
 * pET32 TEV Not Induced || 11.25 || 0.72 ||