Exploiting+recombination+in+single+bacteria+to+make+large+phage+libraries+paper

paper makes use of pDAN plasmid

temp: https://docs.google.com/file/d/0B55PHkbittziSTlkSlhoeGhfWGM/edit

Annotated version of document "C:\kurt\storage\CIM Research Folder\DR\2012\9-3-12\Exploiting (annotated) recombination in single bacteria to make large phage antibody libraries.pdf"

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Interesting Notes

diversity of 3 x 10 11.

To use Cre recombinase to shuffle V genes in the scFv format, a lox site must be placed between the VH and VL genes, which requires a translated lox site as a protein linker.

These results indicate that recombination induced by Cre recombinase can be used to shuffle VH and VL genes between different phagemids,

mixed Vh and Vl scFv still bind antigens well

they use V BASE (immunoglobulin V gene databse) to id the heavy and light chain genes