Primers+Used+for+1st+Construction+of+scFv+Genes

NotI Site: GCGGCCGC SfiI Site: GGCCCAGCCGGCC


 * MHV_BACK1 || CATAT GGCCCAGCCGGCC ATGGCCGATGTGAAGCTTCAGGAGTC ||
 * MHV_BACK2 || CATAT GGCCCAGCCGGCC ATGGCCCAGGTGCAGCTGAAGGAGTC ||
 * MHV_BACK3 || CATAT GGCCCAGCCGGCC ATGGCCCAGGTGCAGCTGAAGCAGTC ||
 * MHV_BACK4 || CATAT GGCCCAGCCGGCC ATGGCCCAGGTTACTCTGAAAGAGTC ||
 * MHV_BACK5 || CATAT GGCCCAGCCGGCC ATGGCCGAGGTCCAGCTGCAACAATCT ||
 * MHV_BACK6 || CATAT GGCCCAGCCGGCC ATGGCCGAGGTCCAGCTGCAGCAGTC ||
 * MHV_BACK7 || CATAT GGCCCAGCCGGCC ATGGCCCAGGTCCAACTGCAGCAGCCT ||
 * MHV_BACK8 || CATAT GGCCCAGCCGGCC ATGGCCGAGGTGAAGCTGGTGGAGTC ||
 * MHV_BACK9 || CATAT GGCCCAGCCGGCC ATGGCCGAGGTGAAGCTGGTGGAATC ||
 * MHV_BACK10 || CATAT GGCCCAGCCGGCC ATGGCCGATGTGAACTTGGAAGTGTC ||
 * MHV_FOR1 || GCCTCCACCTGCAGAGACAGTGACCAGAGT ||
 * MHV_FOR2 || GCCTCCACCTGAGGAGACTGTGAGAGTGGT ||
 * MHV_FOR3 || GCCTCCACCTGAGGAGACGGTGACTGAGGT ||
 * MHV_FOR4 || GCCTCCACCTGAGGAGACGGTGACCGTGGT ||
 * MKV_BACK1 || TCTGGCGGTGGCGGATCGGATGTTTTGATGACCCAAACT ||
 * MKV_BACK2 || TCTGGCGGTGGCGGATCGGATATTGTGATGACGCAGGCT ||
 * MKV_BACK3 || TCTGGCGGTGGCGGATCGGATATTGTGATAACCCAG ||
 * MKV_BACK4 || TCTGGCGGTGGCGGATCGGACATTGTGCTGACCCAATCT ||
 * MKV_BACK5 || TCTGGCGGTGGCGGATCGGACATTGTGATGACCCAGTCT ||
 * MKV_BACK6 || TCTGGCGGTGGCGGATCGGATATTGTGCTAACTCAGTCT ||
 * MKV_BACK7 || TCTGGCGGTGGCGGATCGGATATCCAGATGACACAGACT ||
 * MKV_BACK8 || TCTGGCGGTGGCGGATCGGACATCCAGCTGACTCAGTCT ||
 * MKV_BACK9 || TCTGGCGGTGGCGGATCGCAAATTGTTCTCACCCAGTCT ||
 * MKV_FOR1 || GTTCTT GCGGCCGC CCGTTTCAGCTCCAGCTTG ||
 * MKV_FOR2 || GTTCTT GCGGCCGC CCGTTTTATTTCCAGCTTGGT ||
 * MKV_FOR3 || GTTCTTGCGGCCGCCCGTTTTATTTCCAACTTTG ||

MLV_BACK TCTGGCGGTGGCGGATCGCAGGCTGTTGTGACTCAGGAA
 * MLV_BACK || CAGGCTGTTGTGACTCAGGAA ||
 * MLV_FOR || GTTCT GCGGCCGC CTTGGGCTGACCTAGGACAGT ||

Linker light sequence TTCAGGCGGAGGTGGCTCTGGCGGTGGCGGATCG

Linker heavy sequence CAGAGCCACCTCCGCCTGAACCGCCTCCACCTGAGGAGAC

MHV_BACK_SfiI: CATATGGCCCAGCCGGCCA

MKV_FOR_NOTI: GTTCTTGCGGCCGCCCGT

MLV_FOR_NOTI: GTTCTGCGGCCGCCTTGG