smc_fus+primer

smc_fus

CACTGCACCCAGAGCCATTG Tm of 55.8 20 bp

smc_fus+pSMART2IF forward screening primer produce an approximately 307 bp fragment if the smc1afs sequence is oriented in the forward orientation 307 bp sequence TCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCAAGCAGTGGTATCAAGCGAGGCTTACTGTACACCCGGCTTGCACTGCGCTATTTCCCGCGCGGGCTAACCTCGTTCCCAGGAGTAAGGCGCGGCCTTTCCTACGCCAGCGCCGCTGTCATGGGTTTCCTGAAACTGATTGAGATCGAGAACTTTAAATCGTACAAGGGTCGACAGATTATCGGACCATTTCAGAGGTTTACCGCCATCATTGGACCCAATGGCTCTGGGTGCAGTG

smc_fus+pSMART2IF reverse sequencing primer produce an approximate 363 bp fragment when the smc1afs is in the vector in the reverse orientation 363 bp fragment CACTGCACCCAGAGCCATTGGGTCCAATGATGGCGGTAAACCTCTGAAATGGTCCGATAATCTGTCGACCCTTGTACGATTTAAAGTTCTCGATCTCAATCAGTTTCAGGAAACCCATGACAGCGGCGCTGGCGTAGGAAAGGCCGCGCCTTACTCCTGGGAACGAGGTTAGCCCGCGCGGGAAATAGCGCAGTGCAAGCCGGGTGTACAGTAAGCCTCGCTTGATACCACTGCTTCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGG

products from some primer pairs -smc_fus plus SMC1-mou-FWD

pSMART2IF vector with SMC1afs sequence in forward orientation

pSMART2IF vector with SMC1afs sequence in reverse orientation

"F:\kurt\storage\CIM Research Folder\DR\2013\6-16-13\smc1fs screen\psmart2IF with smc1afs rc.SPF" or "F:\kurt\storage\CIM Research Folder\DR\2013\6-16-13\smc1fs screen\psmart2IF with smc1afs rc.SPF"