see+DNA+incubation+time+11-14-12

Does it make a difference to pipette the cells with DNA, leave the DNA with the cells for 1 min, or use the cells immediately (1-10 s) after adding DNA when doing an electroporation?

From my experience so far the answer is no. On 11-8-12 I did an experiment to test this issue

10 ng DNA 1000 uL SOC 40 min 37 C incubation

1 min sample 7 colonies on 10^0 plate 1.4e4 cfu/ug

Pipetting the DNA 9 colonies on 10^0 plate 1.8e4 cfu/ug

10 s about the same number of colonies as "1 min" and "pipetting", but it was hard to count exactly since the agar was scratched. about 1.5e4 cfu/ug