Pfu

Pfu Polymerase This is a very high fidelity polymerase

last seen in MDR-Rack 4 (bottom row (MDR 49)) in Misc: Plasmid. . box

PCR PROTOCOL FOR PfuTURBO® DNA POLYMERASE on pg. 12 (16 in pdf numbers) of http://stanxterm.aecom.yu.edu/wiki/data/Product_manuals_attach/pfuturbo.pdf

Reaction Mixture

Distilled water (dH2O) 40.6 µl 10× cloned Pfu reaction buffera 5.0 µl dNTPs (25 mM each dNTP) 0.4 µl DNA template (100 ng/µl) 1.0 µlb Primer #1 (100 ng/µl) 1.0 µlc Primer #2 (100 ng/µl) 1.0 µlc PfuTurbo® DNA polymerase (2.5 U/µl) 1.0 µl (2.5 U)d Total reaction volume 50 µl

PCR Cycling Parameters see manual