Camptothecin+Treatment+followed+by+different+abs+and+dyes

In this experiment we treated adherent Balb 3T3 cells with camptothecin overnight (5pm-9am). Later we actually placed the suspended cells in suspension and let them incubate a little longer with camptothecin (about 10am-1pm). Then the cells were treated with different antibodies and dyes.

Caspase, Annexin-V APC, 7-AAD, PI, Caspase + Annexin-V, Caspase + Annexin-V + PI

Here are some pages about the different dyes relevant for this experiment Which dyes can be used together 11-9-11? fluors written on side of instrument

Analysis and Results Second analysis with same axis bmp version "F:\kurt\storage\CIM Research Folder\DR\2013\5-26-13\wiki_download\BMP version_same axis analysis_multi dye test 11-10-11.bmp"