Printing+Actual+Library+010912

Planning to Print Actual cDNA Library 122911

Protocol for Printing cDNA Library 1-9-12

Printing small or individual pools portal 4-11-12

Items 1-21-12

Plate Batches Current well count Current colony count see All Glycerol Stock for Tumor Library 2-17-12
 * Tumor Library Lysate 1-8-12 (2*96+8)
 * Tumor Library Lysate 1-11-12 (3*96+82=370 wells)
 * 4T1 cDNA Library Protein Production in Plate Format for all 3 ligations 1-12-12 (36+60+29 = 125 wells)
 * Protein Production in plate format 1-19-12 (96+7*12+4 = 184)
 * Tumor Library Lysate 1-21-12 ((2*96+12+9=213) + 12 PUC19 rows)
 * Tumor Library Lysate 1-26-12 (96*2+4*12+2 = 242 + 12 pGEX-SMC1fs-27mer wells)
 * Tumor Library Lysate 2-1-12 plate (96*5+12*6+5 =557)
 * Tumor Library Lysate 2-17-12: 1.73e6 colonies (96*17+12*5+11 = 1703)
 * Note that there are some plates that have a date past 2-17-12 (I think it might be 2-21-12 or something). This was just a mistake in labeling. These 2-21-12 plates belong to the 2-17-12 batch.
 * (2*96+8)+(3*96+82)+(36+60+29)+(96+7*12+4)+(2*96+12+9)+(96*2+4*12+2)+(96*5+12*6+5)+(96*17+12*5+11)=3594
 * (2*96+8)*946.8+(3*96+82)*1000+(36+60+29)*1000+(96+7*12+4)*1000+(2*96+12+9)*1000+(96*2+4*12+2)*1000+(96*5+12*6+5)*1000+(96*17+12*5+11)*1000=3.58e6

Cell type used for transformations 8-27-12 Check for Protein 2-13-12 Transfer of Lysate to 384 well plates 2-23-12 Tumor Library Nitrocellulose Print 2-27-12 Approximate Concentrations of Tumor cDNA Library Glycerol Stocks Biomek HiGro

__Map of Experiments__ 4T1 cDNA Library Transformation 1-6-12 4T1 cDNA Library Protein Production in Plate Format 1-7-12 4T1 cDNA Library Protein Production in Plate Format 1-8-12 Single Electroporation 1-9-12 4T1 cDNA Library Protein Production in Plate Format 1-11-12 4T1 cDNA Library Protein Production in Plate Format for all 3 ligations 1-12-12 Five Transformations of A Ligation 1-17-12 Protein Production in plate format 1-19-12 Check for Protein 2-13-12 Colonies on Plates 2-16-12