Cell+Lysate+Print+14

Cell Lysate Print 14 (Grace BioLabs Nitrocellulose Slides; blocking tested with Super G and BSA; primary incubation time tested with 1 hr 37 and 16 hr 23 C) found at 2-11-12

Since the last overnight print run did not work well, we will test another print run 1 hr and overnight with the different blocking buffers.

Setup: 1 slide BSA primary incubation 1 hr 1 slide Super G primary incubation 1 hr 1 slide BSA primary incubation 16 hr 1 slide Super G primary incubation 16 hr

Slide print order in printing machine: the slide closest to the open room was printed first and the other slides were printed in order working back. Johnny said that there was a slight mistake and reprint of about the first 3 spots on the top part of the slides. Hopefully, this will not cause too much of a problem.

Raw Data can be found here: C:\kurt\storage\CIM Research Folder\DR\2012\8-25-12\8-25-12_1140\Printing Library onto array\Cell Lysate Test Prints\blocking and time test 2-11-12 old link "L:\storage\CIM Research Folder\kwhittem\Records in CIM Folder\Categorical Records\Biodesign\Immunosignature to Antigen Bridge Project\Tumor cDNA Library\Printing Library onto array\Cell Lysate Test Prints\blocking and time test 2-11-12"

Summary >> >> >>
 * PDF Summary
 * " F:\kurt\storage\CIM Research Folder\DR\2013\6-22-13\wiki_download\ comparison of blocking buffers and primary incubation time 2-22-12 1.svg"
 * basically The Super G results in less smearing, and the overnight conditions resulted in higher signals. I was able to detect 1/1000 and even 1/10000 SMC1fs dilutions above background.
 * Original Inkscape Files
 * [[file:comparison of blocking buffers and primary incubation time 2-22-12 1.svg]]
 * "F:\kurt\storage\CIM Research Folder\DR\2013\6-25-13\wiki_download\comparison of blocking buffers and primary incubation time 2-22-12 2.svg"
 * "F:\kurt\storage\CIM Research Folder\DR\2013\6-25-13\wiki_download\comparison of blocking buffers and primary incubation time 2-22-12 3.svg"

see also: How to get good results with lysate on nitrocellulose slides