Nested+pcr+screen+scheme+for+frameshift+sequences+in+plasmid+7-31-13

I plan to screen the pools with a forward vector primer + reverse transcript primer. Then I will do the nested pcr with the forward transcript primer followed by the fus primer.

I plan to screen the cDNA with a forward transcript primer + reverse transcript primer. Then I will do the nested pcr with the forward transcript primer followed by the fus primer.

I think I will primarily do these screens with smc and slain

pSMART2IF_for plus slainR (1502 bp) slainF plus slain_fus (362 bp) sequence PCR amplified by slainF and slainR (405 bp)

pSMART2IF_for plus SMC1-mou-RV (163 bp) smc_fus plus SMC1-mou-FWD (124 bp) Primers for amplifying SMC1fs (162 bp)