Phage+Experiments+Portal+060612

Cut vector DNA 060612 (actually this experiment will be an experiment to modify my vector so that I have just one SfiI site and just one NotI site in the appropriate locations) Cut Vector with SpeI and NotI 061412 Diluted pcomb3xss modification primers 062212 Modify SfiI and NotI 062512 Add PolyT near Lac Promoter 070612 Add PolyT near Lac Promoter 8-28-12 Add PolyT near Lac Promoter 9-18-12 Plan scFv ligation 10-3-12 Cut to test for ligation 10-15-12 pcomb and rcomp ligation 10-19-12 Ordering primers to verify sequence 10-25-12 Verify ligation of rcomptt frag 10-26-12 Cut and ligate actual scFv 10-30-12 Send plasmid for sequencing 11-1-12 11-5-12 Correct deletion of stop codon 11-27-12 Polymerase system test for polymerization 1-14-13 linearize plasmid 1-23-13 Sequencing results 3-1-13 plans for further sequencing 3-25-13 sequence investigation 5-9-13 reorder certain phagemid sequencing primers