11-5-12+Correct+deletion+of+stop+codon

11-5-12 Correct Deletion of Stop Codon

The stop codon of the pIII protein was deleted while I was making changes, and this needs to be corrected.

see C:\kurt\storage\CIM Research Folder\DR\2012\11-5-12\pcomb\readd stop codon

primers ordered FP1 RP1 FP2 RP2 LP1 LP2

These primers were received and have a date of 11-15-12 store in 4 C Kurt 11-16-12

size of linearized pComb MN should be 4899 size of pComb fragment to correct deletion of stop codon should be 139 bp

fragment to in-fuse into vector is stored at -20 (these are gel extracted fragments) fragment UV 11-16-12 fragment no UV 11-16-12 fragment min UV 11-16-12

The PCR products necessary to create these gel extracted fragments are also stored at -20 C PCR Product 11-16-12 See notebook with corresponding date for details