Cell+Lysate+Print+13

Cell Lysate Print 13 (Grace BioLabs Nitrocellulose Slides 16 hr room temperature incubation; one with Super G blocking buffer one without) Slides ran around 1-20-12

Spacing of Spots when printed: 500 um

Primary: Balb/Nat SMC1 27 mer Secondary: goat anti mouse

Data Location: L:\storage\CIM Research Folder\kwhittem\Records in CIM Folder\Categorical Records\Biodesign\Immunosignature to Antigen Bridge Project\Tumor cDNA Library\Printing Library onto array\Cell Lysate Test Prints\BSA Super G Test 1-21-12

2-7-12 I was going to align and analyze these arrays, but after looking at the images of the slides, I see that the spots were very faint and quite smeared. Did the overnight condition cause these undesirable results? Johnny and I will print 4 more slides for a test.

1 slide BSA primary incubation 1 hr 1 slide Super G primary incubation 1 hr 1 slide BSA primary incubation 16 hr 1 slide Super G primary incubation 16 hr