Tumor+Library+Glycerol+Stock+Plates+1-6-12

These are glycerol stocks from an overnight culture of 4T1 tumor cDNA library transformation culture. Many different 200 uL wells (2499 wells) containing 78.9 colonies per well on average were grown overnight (some of these may have been lost). These wells were then combined so that there was 946.8 cfu per well on average into 2 plates and 8 wells. The glycerol stock was prepared by taking 62.5 uL of 40% glycerol and 37.5 uL of culture (the Biomek was used to do this). The plates were then placed at -80 C.

The original transformation efficiency of these cultures was 9.8*10^5 cfu/ug.