tumor+antigens+nitrocellulose+array+search+4-25-12

tumor antigens nitrocellulose array

Evaluation of humoral response to tumor antigens using recombinant expression-based serological mini-arrays (SMARTA) Moscow State University SMARTA (serological mini-arrays of recombinant tumor antigens) multianalyte microspot immunoassays they had about 7mm spots with 10^3 to 10^6 pfu of phage per spot

Diagnostic Markers of Ovarian Cancer by High-Throughput Antigen Cloning and Detection on Arrays by Barbara Ann Karmanos Cancer Institute in Detroit Michigan they use the immune system as a biosensor they biopan an ovarian cancer phage display library using sera from a cancer patient they had protein macroarrays containing 480 selected antigen clones

Identification of tumour antigens by serological analysis of cDNA expression cloning by The Nottingham Trent University in the UK quote: To date, over 2,000 tumour antigens have been identified from a variety of malignancies using SEREX. they talk about antigen-specific cancer vaccines SEREX was developed by Pfreundschuh and his colleagues Sahin and Tu¨ reci SEREX generally uses lambda phage expression vectors More than 2,000 antigens have been listed in the SEREX database [29]. Perhaps the SEREX database is now part of the Cancer Immunome Database? http://ludwig-sun5.unil.ch/CancerImmunomeDB/ SEREX has identified mutated gene products as well as splice variants cancer/testis (CT) antigens. note: Since CT antigens are immunogenic and highly restricted to tumours, their discovery has led directly to the development of antigen-specific cancer vaccines this is an interesteing antigen to me: quote: Since CT antigens are immunogenic and highly restricted to tumours, their discovery has led directly to the development of antigen-specific cancer vaccines expressed in cancer tissues compared with matched normal tissues quote: After screening at least half a million clones per library, multiple reactive clones were identified in each library. quote: We have developed an array technique which can beused to rapidly analyse humoral response of multipleSEREX antigens using allogeneic sera. One microlitre ofmonoclonal positive phages (200–300 pfu/ll) is spottedside by side with nonrecombinant phages directly ontothe agar plate premixed with host cells. Following anovernight incubation, ‘‘phage array’’ is transfered ontonitrocellulose membrane and screened with preabsorbedand diluted allogeneic sera [19]. they do pre-absorption of their sera with E.coli and/or phage lysate they state it would be better to develop eukaryotic expression systems for SEREX

Microarrays of tumor cell derived proteins uncover a distinct pattern of prostate cancer serum immunoreactivity by Van Andel Research Institute in Grand Rapids Michigan, Johns Hopkins in Maryland and NovoDynamics in Michigan they use prostate cancer sera against 2D liquid chromatography purified prostate cancer proteins (1760 fractions) with selected proteins, they could classify samples with 98% accuracy they performed heirarchical clustering of their data with their method they cannot identify the proteins and they just refer to the high antibody binders by their fraciton id

Tumor-associated Antigen Arrays for the Serological Diagnosis of Cancer* from the Scripps research Institute they specifically choose the tumor associated antigens beforehand they have a nice table of candidate TAAs recognized by autoantibodies from prostate cancer patients

I still need to check this paper Serologic analysis of ovarian tumor antigens reveals a bias toward antigens encoded on 17q