High+throughput+proteomic+strategies+for+identifying+tumour-associated+antigens.+paper

High throughput proteomic strategies for identifying tumour-associated antigens. paper

Gunawardana CG, Diamandis EP.

Cancer Lett. 2007 Apr 28;249(1):110-9. Epub 2007 Feb 15. Review. 2007 Department of Pathology and Laboratory Medicine, Mount Sinai Hospital, Toronto, Ont., Canada.

article is accessible "C:\kurt\storage\CIM Research Folder\DR\2013\1-26-13\some_serex_papers\High throughput proteomic strategies for identifying tumour-associated antigens..pdf"

Notes High throughput proteomic strategies for identifying tumour-associated antigens

University of Toronto Canada 2007 Cancer Letters Elsevier

Serological analysis of recombinant cDNA expression libraries (SEREX)

q The development of SEREX [8] in the 90s offered a high-throughput approach to analyze the humoral response against TAAs in cancer patients.

pros avoid artifacts of cultured cells

con antibodies to non-tumor associated antibodies

q A database (http://www2.licr.org/CancerImmunomeDB/), Cancer Immunome Database, contains all the autoantigens identified by SEREX.

SERPA 2D western blot -> mass spec

pro fast post-translational modifications

con limited material co-migrating proteins issues with reproducibility

protein microarrays

q ‘‘immunome’’ of a cancer

biased approach (with known analytes) (autoantibody or autoantigen)

Invitrogen protoArray prospector

unbiased approach (new analytes can be discovered) (phage display or cell lysate)

Invented in 1985 by Smith [21], phage display is a powerful proteomic tool used to express proteins or domains of proteins.

pro high throughput a lot of data

con cost

pattern recognition software

q The Luminex xMAP technology appears to be very promising as 100 or more analytes can be studied in one sample alone.

^this is an automated ELISA system