Titration+of+Secondary+Antibody+4-19-12

experiment performed on 4-17-12

Image Files can be found here L:\storage\CIM Research Folder\kwhittem\Records in CIM Folder\Categorical Records\Biodesign\Immunosignature to Antigen Bridge Project\Tumor cDNA Library\Application of sera onto array\sec titr 4-18-12 or https://imtiewhl.livedrive.com/files/4424 or S:\Research\Cancer_Eradication\Discovering tumor specific antigens\Tumor cDNA Library\Application of sera onto array\sec titr 4-18-12

Analysis

These results did not turn out very well. The correlation coefficient between samples is not very good, and the 0.1 nM secondary sample has a lower intensity than the 0.01 nM sample. Fortunately, these samples do have a lower intensity than the 5 nM samples. I think the cause of these unexpected results was that the single chamber frames had very old rubber gaskets which do not seal well and cause leaking to occur. This leaking resulted in several slides breaking when the chamber was opened, and streaks on the slides also made it so that many spots had to be marked as bad rather than analyzed.

Secondary Titration Summary 4-30-12 "S:\Research\Cancer_Eradication\Discovering tumor specific antigens\Tumor cDNA Library\Application of sera onto array\sec titr 4-18-12\analysis\Secondary Titration Summary.docx"

Secondary Titration Intensities

Correlations 2 0.01 nM Samples 0.0802

3 5 nM Samples 0.0.323 r^2 of 5_nM_2 with 5_nM_3 0.0596 r^2 of 5_nM_2 with 5_nM_1

Sample Intensities