Details+of+scFv+Construction+7-7-10

Details of scFv Construction 7-7-10 Primer Sequences Location of Tien's phage info: smb://peptide.biodesign.asu.edu/research/CIM/Mol_Biol_Tech/Phage%20display// Location of Primer Order Form: smb://peptide.biodesign.asu.edu/research/CIM/Mol_Biol_Tech/Phage%20display/phage%20display%20library/primer%20order/Invitrogen_ Oligo Order_042209.xls Source of Primer Sequences: Optimization of primer sequences for mouse scFv repertoire display library construction Note that the first part of the sequences do not correspond to the sequences of the primers that Tien ordered because the first part of their sequences matched with the pHEN1 vector whereas we are using the pCANTAB5 vector. The last part of the sequence matches though.

What are the sequences of the mouse immunoglobulin genes? 10-26-10 The Zhou paper constructed their primers to match the sequences in the Kabat et al database. Specifically they used the sequences from Sequences of Proteins of Immunological Interest 4th edition 1987 pg. 508-553. I have found several sources of antibody sequences. THE INTERNATIONAL IMMUNOGENETICS INFORMATION SYSTEM http://imgt.cines.fr/ [] NCBI IgBlast [] http://www.kabatdatabase.com/index.html I may want to look at the following paper: [|Accessing the Kabat antibody sequence database by computer] I checked out the following book from the library: Sequences of Proteins of Immunological Interest 5th edition 1991