Preparing+Complete+Tissue+Culture+Media

Preparing Complete Tissue Culture Media

Add 100X penicillin/streptavidin to media. Add 10 mL to 1000 mL media. Add 100 mL FBS (fetal bovine serum) to 1000 mL media for 10% FBS

New media last seen in 4 C walk in fridge penicillin/streptavidin last seen in -20 fridge in tissue culture room FBS last seen in -20 fridge in tissue culture room

Some media contains sodium pyruvate. According to wikipedia (http://en.wikipedia.org/wiki/Sodium_pyruvate), sodium pyruvate is commonly added to cell culture media as an additional source of energy, but may also have protective effects against hydrogen peroxide.