forum+response+3-22-12.1640

We have just started doing ELISPOTS with protein instead of peptides. Should we be allowing more time for the cells to respond to allow for antigen processing and presentation? If so how long should we incubate the cells for? Thanks for your help. ||
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  **Date** Wed Dec 19 2007 18:16   **Author** Peter Mellings (petermellings@hotmail.com)   || J Immunol Methods. 1997 Dec 29;210(2):167-74. ||  ||
 * ||  **Subject** [|protein vs.peptide]
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 *  Yes. Insted of 20 hours at least 40 hours. There is a paper out by Carmen Scheibenbogen's group in JIM: Schmittel A, Keilholz U, Scheibenbogen C. Evaluation of the interferon-gamma ELISPOT-assay for quantification of peptide specific T lymphocytes from peripheral blood.