Basic+plan+for+random+hexamer+library+construction+1-24-13

I will construct the library using four different methods and then compare the complexity of each method.

for primers used see cDNA Library Random Hexamer Plan 12-12-12

Synthesize 1st strand cDNA from poly A purified RNA using reverse transcriptase in In-Fusion SMARTer Directional cDNA Library Construction Kit -> 2nd strand synthesis with PrimeStar Synthesize 1st strand cDNA from poly A purified RNA using reverse transcriptase in In-Fusion SMARTer Directional cDNA Library Construction Kit -> 2nd strand synthesis with dynazyme

Synthesize 1st strand cDNA from poly A purified RNA using PrimeScript reverse transcriptase -> 2nd strand synthesis with PrimeStar Synthesize 1st strand cDNA from poly A purified RNA using PrimeScript reverse transcriptase -> 2nd strand synthesis with dynazyme