Test+of+Decanting+after+fluor+is+added+2-3-12

Experiment performed on 2-3-12

Decanting the supernatent to get rid of unbound antibody definitely does seem to make a difference. The decanted sample had a lower intensity on the histogram plot than the non-decanted sample. However, neither of these negative control samples had intensity as high as an actual sample with CD8 T cells. See summary below for more details.

Files for this experiment can be found here: L:\storage\CIM Research Folder\kwhittem\Records in CIM Folder\Categorical Records\Biodesign\Age Associated Stem Cell Autoimmunity\CTL Assay\Experiments\cd8 neg decant test 2-3-12 or https://imtiewhl.livedrive.com/files/1354

Antibody used: CD8-PE PE uses FL2

Summary >> >> >>
 * Inkscape Files
 * "F:\kurt\storage\CIM Research Folder\DR\2013\6-16-13\wiki_download\Summary 1 2-7-12.svg"
 * "F:\kurt\storage\CIM Research Folder\DR\2013\6-16-13\wiki_download\Summary 2 2-7-12.svg"
 * [[file:Summary 3 2-7-12.svg]]
 * PDF File
 * "F:\kurt\storage\CIM Research Folder\DR\2013\6-16-13\wiki_download\CD8 neg decant summary 2-7-12 (1).pdf"