Cell+lysate+printing+10

spots were spaced farther apart (800 um) on nitrocellulose slides; this distance was great enough to keep the spots apart Date Slide was run in Tecan: around 11-6-11

In this run, I increased the concentration of the primary antibody from 1:500 to 1:20. I also increased the incubation time of the primary from 1 hr to 2hr. I was able to detect much lower dilutions of protein lysate than I ever have before. The slide did not have an even intensity though. I think the primary concentration may have been to high. Perhaps I could even do a 1:500 dilution of primary if I did an overnight incubation. In the future, I may want to try a range of primary concentrations and times.

Whole Array

Block 1

Block 2 Block 1 Graph Block 2 Graph