progress+towards+sequencing+tmem+fragment+6-25-13

Before I perform 96 minipreps on one of the thap2+tmem19 ( TR_E10339) positive pools in the 1-41 pool set, I need to see if the sequence I have would produce the same peptide that Shen has sera against.

According to the table found here

"F:\kurt\storage\CIM Research Folder\DR\2013\6-19-13\chim transc\table of results 6-19-13.xlsx" 10339 was positive in pools 8, 19, and 20

I performed a PCR with dynazyme with 10339 primers with 8, 19, and 20 pools