determining+concentration+of+dna

Nanodrop

Does glycogen absorb in the DNA range? Not really very much. I did a test measurement of a 10 uL sample containing 2 uL of 20 ug/uL glycogen and the result was 12.3 ng/uL with a 260/280 of 2.14. The curve also did not have a very high well shaped peak. Also, the SMARTer In-Fusion directional library kit instructs the user to nanodrop a sample after an ethanol precipitation with glycogen has been performed