Tumor+Antibody+Dot+Blot+8-22-12

My goal was to redo the previous dot blot. This time I planned to do the naive at 10e6 fold dilution, do replicates of spots, and have a membrane for naive, tumor, secondary, and no antibody.

Raw Results L:\storage\CIM Research Folder\DR\2012\8-23-12\dot blot\raw results

Summary of results "L:\storage\CIM Research Folder\DR\2012\8-23-12\dot blot\Tumor Ab Dot Blot Summary 8-22-12.docx"

I don’t think the results from this experiment are valid because the background intensity of each membrane varies drastically depending on how clean the container was that the membrane was incubated in. Some of the containers even had some residual blue stain (most likely coomassie stain) which stained my membranes blue (“No ab” and “Naïve ab” membranes). I think I will repeat this experiment using brand new empty tip boxes which should all be just as clean as one another.