Preparation+of+a+Linearized+Vector+by+PCR

PrimeStar Max polymerase seems to work the best for vector linearization

see 11-27-12 Polymerase system test for polymerization for initial test of polymerases for linearization

1 ng of template seems to work just fine see 11-27-12 of notebook for an example

Advantage HD can also be used, but doesn't seem to work as well

see "C:\kurt\storage\CIM Research Folder\DR\2012\11-5-12\preparation of a linearized vector by PCR 11-5-12.docx"

The In-Fusion SMARTer Directional cDNA Library Construction Kit also has instructions for linearization.