Discussion+with+Mitch+Magee+11-21-11


 * Methods for purifying T cells
 * nylon wool option gets rid of B cells and adherent cells
 * need to add APCs back (like macrophage cells)
 * mouse ficoll gradient
 * antibody and complement; anti-CD4 rabbit complement
 * don't use trypsin because it can strip away cell surface markers
 * count cells at every step
 * prime protein boost before harvesting spleen
 * 3-7 days before we harvest
 * wash mitomycin C away several times
 * test Robosep system
 * FACS analysis with CD8 and CD3 (make sure CD4 is not present
 * good target cell line: Balb mouse macrophage cell line (RAW (7.1?))
 * put in name of magnification in images
 * put scale bar on images
 * find out size of T cell