Plan+for+modifying+pComb3XSS+vector+061312

found here:

C:\kurt\storage\CIM Research Folder\DR\2012\6-13-12 description of changes actually made (not just plans) can be found here "C:\kurt\storage\CIM Research Folder\DR\2012\6-13-12\changes actually made as of 11-5-12\Modifying pComb3XSS vector.docx"

Here is the sequence of the pcomb after the NotI site has been modified (the 1st In-fusion modification reaction) "C:\kurt\storage\CIM Research Folder\DR\2012\7-26-12\pcomb sequence analysis\pcomb_MN.txt" This sequence will be pComb3XSSv2 (I will no longer try to add the poly T site near the LacI) <- Note on 10-2-12 last seen at pComb MN 10-18-12 (MN is for modified NotI) at 9-23-12 KW -20 C

7-29-12 My first in-fusion reaction to modify the NotI site worked. However, my 2nd in-fusion reaction to add the poly Ts near the Lac site did not work, and Andrey found that some of the primers were wrong. I'll need to redesign these primers. see "C:\kurt\storage\CIM Research Folder\DR\2012\6-13-12\inserting polyT site corrected.svg"

Oligo Order "C:\kurt\storage\CIM Research Folder\DR\2012\7-29-12\pcomb\Oligo Order Kurt 7-29-12.xls"

8-28-12 Location of some primers

Stock primers stored in "10-3-11 KW contains some phage stuff -20 C box" Working primers stored at "4 C box 082510 dot 1020"

RPTFC RPTFC2B RPTFC2 RPTFCB SAPTFC SAPTRC

11-5-12 Correct deletion of stop codon