Gel+extraction+of+SMC+related+bands+6-24-13

These bands were gel extracted on 6-22-13

Gel image here "S:\Research\Cancer_Eradication\Discovering tumor specific antigens\Tumor cDNA Library\6-22-13\chimeric transcript gel 0p7 exp inverted 6-22-13.jpg"

Quantification of bands 6-24-13d1006

Now that I have quantified the bands I can figure out how to setup the pJET cloning reaction.

Need to know how much DNA corresponds to 0.15 pmol ends for all 3 bands

0.15 pmol ends of top band. I would say the top band is around 460bp (0.15*"pmol"*"ends")*(1*"mol"*"ends")/(1e12*"pmol"*"ends")*(6.02e23*"ends")/(1*"mol"*"ends")*(1*"molecule")/(2*"ends")*(460*"bp")/(1*"molecule")*(1*"mol"*"bp")/(6.02e23*"bp")*(660*"g")/(1*"mol"*"bp")*(1e9*"ng")/(1*"g")*(1*"uL")/(1.8*"ng")= 12.7*"uL" 0.15 pmol ends of middle band. I would say the middle band is around 160 bp (0.15*"pmol"*"ends")*(1*"mol"*"ends")/(1e12*"pmol"*"ends")*(6.02e23*"ends")/(1*"mol"*"ends")*(1*"molecule")/(2*"ends")*(160*"bp")/(1*"molecule")*(1*"mol"*"bp")/(6.02e23*"bp")*(660*"g")/(1*"mol"*"bp")*(1e9*"ng")/(1*"g")*(1*"uL")/(0.8*"ng")=9.9*"uL" 0.15 pmol ends of bottom band. I would say the middle band is around 140 bp (0.15*"pmol"*"ends")*(1*"mol"*"ends")/(1e12*"pmol"*"ends")*(6.02e23*"ends")/(1*"mol"*"ends")*(1*"molecule")/(2*"ends")*(140*"bp")/(1*"molecule")*(1*"mol"*"bp")/(6.02e23*"bp")*(660*"g")/(1*"mol"*"bp")*(1e9*"ng")/(1*"g")*(1*"uL")/(0.6*"ng")=11.6*"uL"