Preparing+Tumor+cDNA+and+anti-SMC1fs+Ab+slide+5-19-13

Preparing Tumor cDNA and anti-SMC1fs Ab timeline of mouse immunization timeline of tumor injection timeline of SMC1fs injection

"F:\kurt\storage\CIM Research Folder\DR\2013\5-19-13\Preparing Tumor cDNA and anti-SMC1fs Ab.pptx"

-content on slide { Information about injection details can be found here http://www.azim58.wikispaces.net/Injection+of+mice+with+tumor+cells+and+SMC1fs

Timelines can be found here L:\storage\CIM Research Folder\kwhittem\Records in CIM Folder\Categorical Records\Biodesign\Immunosignature to Antigen Bridge Project\Tumor cDNA Library\tumor injection\timeline

Tumor injection = Injected 7000 4T1 Cells (mice were 42 days old at this point) Genetic Immunization = (1 shot per ear with each shot consisting of 1 ug SMC1 wt+fs/pcMVi-rCOMTT and 1 ug CpG2395) Protein boost = (5 ug SMC1fs fused with GST along with Alum) Peptide boost = (5 ug peptide per mouse) }