insert+to+vector+ratios+with+ligation

Ligation vector and insert ratios

lab manuals usually say "molar ratio 1:3 vector: insert" http://www.methods.info/Methods/RNA_DNA/ligation_simple.html

In most cases either a 1:1 or a 1:3 molar ratio of vector:insert works well. http://www.mcdb.ucla.edu/Research/Arispe/Protocols/Vector_Insert_Ratio.pdf

Here's a calculator for the insert to vector ratio problem http://www.promega.com/techserv/tools/biomath/calc06.htm

Reasoning for vector to insert ratios

While if you increase the amount of insert, there is higher chance it gots incorporated. http://www.biology-online.org/biology-forum/about19798.html