Dynazyme+Protocol

extension time for dynazyme:

This is from the excel spreadsheet attached below. The amount of primers reflect the amount for a primer concentration of 20uM. If the primer is a different concentration other than 20uM, change the volumes or dilute primer down to said concentration.

Protocol Here "C:\kurt\storage\CIM Research Folder\DR\2013\2-26-13\dynazyme\pcr_protocol\Dynazyme PCR.xls"

In our lab we use Dynazyme II DNA polymerase

I have some aliquots of dynazyme here: 7-2-13d1700 -20 C box

official manual here http://www.thermoscientificbio.com/uploadedFiles/Resources/f503sl_dynazyme2_prodinfo_A4_TS_v1_low.pdf and here "F:\kurt\storage\CIM Research Folder\DR\2013\2-26-13\dynazyme\dynazyme II dna polymerase product information.pdf"

see also Thermocycle Conditions portal 5-9-13

see also Dynazyme Colony PCR Protocol see also comparison of primestar max with dynazyme 7-3-13