Protein+boost+1-10-12

1 mL PBS was added to SAS adjuvant. Ovalbumin protein was diluted to a concentration of 0.1 mg/mL. 500 uL of SAS solution and 500 uL of 0.1 mg/mL protein were mixed together (so this was a 1:1 mixture composed of SAS and 0.05 mg/mL protein). The protein SAS solution was injected into the mice intraperitoneally. 3 mice received 200 uL, and 2 mice received 150 uL