Sequencing+primers+for+the+In-Fusion+SMARTer+Directional+cDNA+Library+Construction+Kit

Sequencing primers that came with kit

Forward Screening Primer (closest to promoter; 50 bp from end of forward screening primer to beginning of in-fusion site) TCACACAGGAAACAGCTATGA Reverse Screening Primer (furthest from promoter; 106 bp from end of reverse screening primer to beginning of in-fusion site) CCTCTTCGCTATTACGCCAGC

see alignment of vector with sequencing primers "C:\kurt\storage\CIM Research Folder\DR\2013\2-11-13\pSMART2IFD_sequencing\pSMART2IFD_with_sequencing_primers.SPF"

I would prefer sequencing primers that are 200 bp upstream of the site to be sequenced. Here will be my new custom primers. reverse sequencing primer (SDSR for pSMARTIFD Sequencing reverse) ACCGCACAGATGCGTAAGG 19 bp 53.1 Tm

forward sequencing primer (SDSF for pSMARTIFD sequencing primer forward) AATGCAGCTGGCACGACAGG 20 bp 55.8 Tm

The SDSF and SDSR primers are located at 4 C Kurt 11-16-12 (2.5 uM and 10 uM) (the 100 uM stock is located -20 C freezer Q Reese Probe 80)

Instances of new custom sequencing primers for the in-fusion SMARTer Directional cDNA Library Construction Kit 2-11-13 Primer Order Form "C:\kurt\storage\CIM Research Folder\DR\2013\2-11-13\pSMART2IFD_sequencing\Primer Order Form Kurt 2-11-13.xls" Product Slip "C:\kurt\storage\CIM Research Folder\DR\2013\2-14-13\sequencing primers\Sequencing primers for pSMARTIFD.pdf"