Orientation+of+Peptides+Synthesized+onto+Tentagel+Beads

The peptides are synthesized on the tentagel resin from the C terminus

Note that on the version 2 arrays the N terminal side of the peptide is attached to the array surface (protein sequences are typically written from the N to C terminus). On the version 1 arrays the C terminal side of the peptide is attached to the array surface. Selecting peptides from the version 2 array to be attached to the Tentagel beads is not a straightforward process. The peptide sequence cannot just be simply reversed so that the farthest amino acids on the array are also the farthest amino acids from the resin. When this is done, the type of link between each amino acid will be reversed, and the peptide may not be exactly the same. Although many antibodies and other molecules may interact with the functional groups of the amino acids in the same way there is no guarantee. Therefore, in order to obtain exactly the same type of peptide on the tentagel resin as that found on the array the peptides must be synthesized, purified, and then conjugated to the tentagel resin.