Alternative+CTL+Assays+to+CRA

Alternative CTL Assays to CRA Note: Not all of the papers here are about caspase.

Flow cytometry and apoptosis detection notes/paper

Measurement of CTL-Induced Cytotoxicity The Caspase 3 Assay (2003)



Multiparametric Flow Cytometry 2007

A sensitive flow cytometry-based cytotoxic T-lymphocyte assay through detection of cleaved caspase 3 in target cells (2005) []

 Assessment of Lymphocyte-Mediated Cytotoxicity Using Flow Cytometry (2004) http://www.springerlink.com/content/j8v4l4gp83241442/#section=98733&page=1 ^^Vanessa tried reading this paper but it only lets me read the first 3 pages and won't let me download the PDF. Where you able to get more than that from this??

Visualization and quantification of cytotoxicity mediated by antibodies using imaging flow cytometry (2011) http://www.sciencedirect.com.ezproxy1.lib.asu.edu/science/article/pii/S0022175911000585 Note: This paper seems to focus more on antibody dependent cellular cytotoxicity.

Development and application of a multiplexable flow cytometry-based assay to quantify cell-mediated cytolysis (2010) http://onlinelibrary.wiley.com.ezproxy1.lib.asu.edu/doi/10.1002/cyto.a.20887/abstract;jsessionid=BC08B53F2E7D099049D34E46A0267D3A.d01t02 Note: This paper seems to focus on the quantitative aspects of cell-mediated cytolysis

Application of a Flow Cytometric Cytotoxicity Assay for Monitoring Cancer Vaccine Trials (2009) http://ovidsp.tx.ovid.com.ezproxy1.lib.asu.edu/sp-3.4.2a/ovidweb.cgi?QS2=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 Note: this paper looks quite interesting and very applicable to our situation ^^I couldn't open this either :(

New Flow cytometric assays for monitoring cell-mediated cytotoxicity review paper I may want to look up justification for sensitivity jam of method

This paper was recommended by Blattman Counting Antigen Specific CD8 T Cells A Reevaluation of Bystander Activation during viral infection