PCR+Screen+Portal+11-7-12

Designing primers for PCR screen 11-7-12 Initial PCR Screen 11-26-12 Sequence Analysis 12-3-12 Overnight Culture of Library PCR to screen for selected frameshifts and gene fusions 4-11-13 PCR Screen for SMC1fs 4-19-13 PCR Screen for SMC1fs 4-25-13 PCR Screen for SMC1fs 5-3-13 PCR Screen for 13 validated mouse chimeric transcripts 5-2-13 PCR Screen for 7 chimeric transcripts in 41 pools 5-21-13 Miniprep of pools for SMC1fs screen 5-23-13 Design SMC1fs primers to determine orientation and sequence 6-14-13 Gel extraction of SMC related bands 6-17-13 More PCRs for 7 chimeric transcripts in pools 1-41 6-19-13 Gel extraction of SMC related bands 6-24-13 progress towards sequencing tmem fragment 6-25-13 investigation of smc related bands 6-26-13 troubleshoot pcr 6-29-13 screen 41 pools for slain 7-10-13 sequencing results 7-17-13 screen of tumor library antibody binding sequences 7-19-13 sequencing results 7-22-13 sequencing results 7-25-13 sequencing results from slain screen 7-28-13 sequencing results from nested pcrs of large pools 7-31-13 nested pcr screen of sample 87 7-31-13 sequencing results from nested pcrs of large pools 8-6-13 - "F:\kurt\storage\CIM Research Folder\DR\2013\8-6-13\sequencing results of nested pcrs of large pools on 8-6-13.txt" summary of pcr screens started in summer 2013 -"F:\kurt\storage\CIM Research Folder\DR\2013\8-7-13\summary of pcr screens started in summer 2013.txt" sequencing results from nested pcrs of pools 8-9-13 -"F:\kurt\storage\CIM Research Folder\DR\2013\8-8-13\smc sequence results 8-9-13" -These sequences were not very good, and I probably had too little material to send for sequencing.