Cell+Lysate+Printing+6

__Different Slide Types will be tested__ Nitrocellulose CodeLink Valiery's Polymer Slides

printed onto Valiery's polymer slides; not much improvement over any other test found at 8-26-11 of notebook

__Things to Keep in Mind__ -Decrease Z-axis speed to <20 mm/sec to avoid nitrocellulose damage -A19 GST-SMC1fs was diluted (1/4 dilution) -A13-A16 were prepared by diluting pGEX-SMC1fs into pET32b non-induced (I ran out of pET32b induced) 8-11-11: Now I will wait for my sample nitrocellulose slides to arrive. 8-24-11: The sample nitrocellulose slides never did arrive. I plan to print onto the other array types anyway tomorrow.

Plate for printing: SMC1fs Dilution Series 8-10-11 Layout of Plate: Non-denatured Samples: A1-A9 and A11-A19 (duplicated) Denatured Samples: C1-C9 and C11-C19 (duplicated)
 * Samples (9): 1X || 0.5X || 0.1X || 0.01X || 0.001X || 0.0001X || 0X || GST || GST-SMC1fs ||