7-AAD+or+PI+protocol


 * 7-AAD or PI protocol**

Before starting: Dilute 10X binding buffer to 1X using distilled water (1mL 10X binding buffer + 9mL dH20)

1. Wash cells once in PBS 2. Wash cells once in 1X binding buffer (prepared above) 3. Resuspend cells in 1X binding buffer at 1-5x10^6/mL 4. Add 5uL of PI staining solution OR 7-AAD staining solution. 5. Analyze by flow cytometry within 4 hours, storing at 2-8C in the dark

Note that to run through our flow cytometer, should add 1X PBS right before analysis so that the final volume is 1mL (as preferred by the cytometer)

Documents