Meeting+with+Stephen+5-12-13

I showed him the data about screening the cDNA library pools for SMC1fs and the chimeric transcripts powerpoint here F:\kurt\storage\CIM Research Folder\DR\2013\5-7-13\Data Update 5-7-13.pptx

-he wanted me to draw a Gantt chart -he mentioned I could compare expression level doing an actin titration to determine level of SMC1fs expression in cell -look at some calculations to see how many transcripts there are per cell -look for the lower band in the top 10 SMC1fs pools <- I did this PCR previously, but at that time I was using different PCR conditions so the lower band may not have been there. -how many transcripts are there in a cell total? -it looks like I will need to screen the array again and use sera that is known to bind to SMC1fs. He thinks Shen might have some of this sera. I could also screen the array using sera against some of the chimeric transcripts. If I make another array, I should include some SMC1fs spots that are not diluted 1/1000