Cell+Lysate+Print+12

Cell Lysate with Super G Blocking Buffer Summary Grace BioLabs Nitrocellulose Slides with Super G Blocking Buffer 2 hr primary at 37C Summary found at 12-19-11

The Super G Blocking Buffer appears to decrease all intensities considerably. However, the ability to distinguish between different lysate dilutions and the ability to detect lower dilutions seems to increase. The error bars of replicate lysate spots also seems quite small.

Location of Data: S:\Research\Cancer_Eradication\Users\kwhittem\kwhittem\Raw Data Often Originally on Research Drive\2011\12-18-11 and here L:\storage\CIM Research Folder\DR\2011\12-18-11

Sera: BALB Nat anti-mSMC1 27 mer Low anti-t Hap2 + Rbrwh Pooled 10/15/11 Some Details of Experiment 12-19-11

Two Dilutions of primary sera were tested on the two different blocks on this slide (upper chamber and lower chamber)

Top Block (1:20 primary dilution)



Bottom Block (1:500 primary dilution) "F:\kurt\storage\CIM Research Folder\DR\2013\5-27-13\wiki_download\Bottom_Block.png" "F:\kurt\storage\CIM Research Folder\DR\2013\5-27-13\wiki_download\Bottome Block Graph 2 12-18-11.png"

Brain Cancer Super G Comparison