Pierce+Silver+Stain+Protocol

Pierce Silver Stain Protocol Item 24612 http://www.piercenet.com/instructions/2161478.pdf Note that this pdf contains more information and a more in-depth protocol than the protocol below


 * Wash gel 2 × 5 minutes in ultrapure water.
 * Make sure to handle the gel with clean gloves or sterile equipment to avoid later smudges on the gel
 * Fix gel 2 × 15 minutes in 30% ethanol:10% acetic acid solution.
 * Wash gel 2 × 5 minutes in 10% ethanol, then 2 × 5 minutes in ultrapure water.
 * Prepare Sensitizer Working Solution (50μL Sensitizer with 25mL water).
 * Sensitize gel for 1 minute, then wash 2 × 1 minute with water.
 * Make sure that any silver stain reagents are discarded in an appropriate silverstain waste container.
 * Prepare Stain Working Solution (0.5mL Enhancer with 25mL Stain).
 * Stain gel for 30 minutes.
 * Prepare Developer Working Solution (0.5mL Enhancer with 25mL Developer).
 * Wash gel 2 × 20 seconds with ultrapure water, then develop gel for 2-3 minutes until bands appear.
 * Stop with 5% acetic acid for 10 minutes.