11-7-12+Carb+plate+test

11-7-12 experiment to test this question I did a test to find out. I plated the same electroporated culture onto plates prepared on 7-26-11 and plates prepared on 11-6-12. The bacteria were electroporated with a linearized vector in-fused with a control insert from the In-Fusion SMARTer Directional cDNA Library Construction Kit. The next morning there were many more colonies on the old plates on the new plates. This may indicate that the Carb in the old plates had degraded. Another possibility is just that the colonies will take much longer to grow on the fresh plates. Image of Plates "C:\kurt\storage\CIM Research Folder\DR\2012\11-7-12\plate image\11-7-12 image of plates.jpg" Top left: 7-26-11 plate 1e-4 dilution Bottom left: 7-26-11 plate 1e0 dilution Top right: 11-6-12 plate 1e-4 dilution Bottom right: 11-6-12 plate 1e0 dilution

I plan to electroporate cells with H20 and plate them onto the two different types of plates (more than 1 year old and new). 11-8-12 Checked plates of cells electroporated with H20. The old plate was completely confluent when it should not have been, and the new plate did not have anything on it just as it should.

picture of plates

"C:\kurt\storage\CIM Research Folder\DR\2012\11-8-12\neg control lb plates\neg control on old and new plates 11-8-12.jpg"

Therefore, carb plates that are 1 yr and 3 months old are too old to use.