Question+about+correct+protocol+with+EasySep+kit+1-4-12

orders@stemcell.com Question about correct protocol with EasySep kit 1-4-12 Hi Stemcell Technologies representative,

I have two different kits that I plan to use with the ROBOSEP machine soon. My kits have catalog numbers

19753A and 19751A

However, the protocols on the instrument have don't have this "A" at the end. I have

Mouse CD8+ T Cell Negative Selection 19753-high purity Mouse CD8+ T Cell Negative Selection 19753-high recovery and Mouse T Cell Negative Selection 19751-high purity (D particles) Mouse T Cell Negative Selection 19751-high recovery (D particles)

I'm pretty sure these protocols should work with these kits even though they don't have the "A". However, I just wanted to double check. Do you know what the "A" is for, and will these kits work with these protocols? Thanks for any information you can offer me!

Best regards, Kurt Whittemore

Graduate Student Arizona State University BIODESIGN INSTITUTE Center for Innovations in Medicine 1001 S McALLISTER AVE TEMPE, AZ 85287

Dear Kurt,

Thank you for contacting STEMCELL Technologies.

19753 or 19751 are the catalog numbers for the kits you have in hand and these correspond to the RoboSep e-protocols you have listed, the e-protocols you have listed are the correct ones to use for these kits. The ‘A’ in 19753A means it is a subpart or component number of those kits. For example, catalog number 19753 contains box 19753A (containing the EasySep components) and 13551 (the Rat serum).

Please note that kit 19753 has also been very recently changed, if you have a kit with a lot number of 11H40542 or higher (eg. 11H40542 or 11J41563) you will need to use a new e-protocol: Mouse CD8 T Cell Negative Selection 19753 (D particles)

If you have one of these lots please let me know and I will be happy to send you the new e-protocol with instructions for how to load it onto your RoboSep unit.

I hope that this information is helpful to you. Should you have any further questions, please contact myself, Technical Support or one of the Technical Sales Representatives for your area, Randy Lauzon (Territory Manager - Cell Separation Products) or Sarah Nahid (Inside Sales).

Sincerely, Amanda

Ah okay. Well that's a simple explanation that makes sense.

Yeah, I've actually already uploaded the new protocol for the 19753 with the "D Particles". Someone from STEMCELL e-mailed me about that a few months ago. Thanks!