Injection+of+mice+with+tumor+cells+and+SMC1fs

It looks like I injected with tumor cells and did a genetic immunization on 5-25-10.

Tumor Injection and Genetic Immunization 5-25-10 7000 tumor cells used With 7000 tumor cells palpable primary tumors develop in 2-3 weeks

amount of tumor cells used and type (4T1, 7000 cells in a 100 uL volume, 7000 cells results in a primary tumor in 2-3 weeks) Note that mouse 5 may have been injected with slightly too many tumor cells and this mouse was the one that ended up with a maximum size tumor the soonest.

Injection information C:\kurt\storage\CIM Research Folder\kwhittem\Records in CIM Folder\Dated Records\5-24-10 Induction of Tumor and Genetic Immunization

4T1 model protocol document Title: Mouse 4T1 Breast Tumor Model from Current Protocols in Immunology "C:\kurt\storage\CIM Research Folder\kwhittem\Records in CIM Folder\Dated Records\5-20-10 Planning and Research\4T1\4T1 model protocol.pdf"

info also on 5-24-10 of notebook

This spreadsheet has more details

Here's the original cage cards

Tumor Volume record sheet Spreadsheet for tumor volumes can be found here: C:\kurt\storage\CIM Research Folder\kwhittem\Records in CIM Folder\Dated Records\6-8-10 Records

Timeline graphics can be found here C:\kurt\storage\CIM Research Folder\kwhittem\Records in CIM Folder\Categorical Records\Biodesign\Immunosignature to Antigen Bridge Project\Tumor cDNA Library\tumor injection\timeline

2-16-13 For the genetic immunization the peptide can be assumed to be the 17aa frameshift part of SMC1fs The protein can be assumed to be the 17aa frameshifted fused to GST

300 psi used for genetic immunization

Titer after genetic immunization can be found here (before and after protein boost): C:\kurt\storage\CIM Research Folder\kwhittem\Records in CIM Folder\Dated Records\7-16-10 Records

Titer before and after peptide boost: C:\kurt\storage\CIM Research Folder\kwhittem\Records in CIM Folder\Dated Records\8-5-10 Records