Background+Info+for+Immunosignature+to+Antigen+Work

My DoD Breast Cancer Predoctoral Traineeship application has some good background information about my project.

"F:\kurt\storage\CIM Research Folder\DR\2013\6-16-13\wiki_download\Identification of Breast Cancer Antigens through Immunosignatures.doc"

"F:\kurt\storage\CIM Research Folder\DR\2013\6-16-13\wiki_download\References Cited.odt"

Here's a basic outline of the paper
 * Career Goals
 * Improve human health
 * Training
 * Courses, mentors, Biodesign environment
 * Peter Lee, Kathryn Sykes
 * Research Project
 * Introduction
 * description of antigen discovery platform
 * 2 reagents
 * antibody-carrying sera
 * random sequence 20 mer peptides
 * SMC1fs positive control
 * eventual cancer vaccine
 * Background
 * tumor associated antigen repressed mammary tumors in rats [9]
 * whole cell tumor lysate was immunogenic [10]
 * self tumor mixtures in clinical trials [11]
 * more antigens would be good
 * more specific antigens needed
 * tumor associated antigen examples: HER2/neu, MUC1, MAGE
 * Specific Antigens: frameshifts, chromosomal rearrangements, aberrant protein modifications
 * example of tumor specific antigen from lymphoma group [13]
 * learn from immune system (from antibodies)
 * random peptide array can distinguish diseases
 * need to discover original antigen
 * SMC1fs positive control
 * SMC1fs found more in cancer than normal cells
 * plan for SMC1fs sera
 * phage display technology
 * small antibodies
 * unlimited amount of phage
 * compare sera and library
 * conclusion
 * Methods
 * Identification of breast cancer specific immunosignature
 * format of slides
 * basic procedure
 * process for determining informative peptides
 * different random arrays could be used
 * Probing biological samples
 * random peptide array helps purify antibodies
 * probe biological space
 * Western blot
 * cDNA library
 * Phage Library Approach
 * Process for making phage library
 * Discovering human antigens
 * Relevance

Cancer vaccine references >>>
 * Vaccination with soluble low-molecular weight tumor-associated proteins suppresses chemically-induced mammary tumorigenesis in rats
 * I wasn't able to get an electronic copy of this paper online normally, but I did get it from the ILLIAD ASU service
 * "F:\kurt\storage\CIM Research Folder\DR\2013\6-16-13\wiki_download\Vaccination with soluble low-molecular weight tumour-associated proteins suppresses Chemically-induced Mammary Tumorigenesis in Rats.pdf"
 * Immunotherapeutic potential of whole tumour cells
 * http://www.springerlink.com.ezproxy1.lib.asu.edu/content/a88nu01yyxxjvyrv/fulltext.pdf
 * Cancer vaccines: between the idea and the reality
 * http://www.nature.com.ezproxy1.lib.asu.edu/nri/journal/v3/n8/pdf/nri1150.pdf

Shen also has some data to show that a cancer vaccine is effective. "F:\kurt\storage\CIM Research Folder\DR\2013\6-16-13\wiki_download\cancer vaccine protection .ppt"

Questions Have I found a paper that shows that scFv bind the same antigen as the original antibody? I think I've read this before, but now I don't know where it is.