Freezing+Tissue+Culture+Mammalian+Cells

Pellet the cells and resuspend in 1 mL freezing media in a cryogenic vial. Place the vial into the cryogenic container containing isopropanol (such a container allows the temperature to drop slowly) and place this container into -80 C overnight. Transfer vial to -150 C for long term storage.

Cryogenic vials last seen in tissue culture room in 2nd or 3rd drawer in dresser near door. Tissue culture freezing media (some amount of DMSO and FBS?)