Chromium+Release+CTL+Assay+with+Ova+8-17-12

8-17-12 Chromium Release CTL Assay with Ova

In this experiment the target cells were resuspended to the wrong volumes by accident, and therefore I did not have the E:T ratios I originally planned. I also did not have enough targets to test the targets without the peptide with the effectors at different ratios. I may repeat the experiment.

Item: ova mouse sera 8-17-12 KW at CTL 6-13-11 KW -20 C

CTL Plate Map L:\storage\CIM Research Folder\DR\2012\8-16-12\ctl plate map

Results C:\kurt\storage\CIM Research Folder\DR\2012\8-18-12\Ova CTL

The results came out basically the way I expected them to. However, the targets alone pulsed with peptide exhibited a lot of killing which I did not expect.

Notes on paper used for plate layout 8-17-17 "L:\storage\CIM Research Folder\DR\2012\8-18-12\Ova CTL\Notes on paper used for plate layout 8-17-12.pdf"