Potential+target+antigens+for+immunotherapy+identified+by+serological+expression+cloning+(SEREX).

Potential Target Antigens for Immunotherapy Identified by Serological Expression Cloning (SEREX) Dirk Jager Germany

PMID = 17172736

paper found here "C:\kurt\storage\CIM Research Folder\DR\2013\1-26-13\some_serex_papers\Potential Target Antigens for Immunotherapy Identified by Serological Expression Cloning (SEREX).pdf"

q the presence of tumor-infiltrating lymphocytes is associated with a better prognosis in individual patients (1–4)

q The first tumor-associated antigen identified with this technique was the melanoma antigen MAGE-1. (no reference was given for this)

T-cell epitope cloning technique

q Mutated antigens are a category of individual antigens; typically, the mutation is not shared by other individuals, with few exceptions such as the k-ras oncogene mutated in pancreatic cancer.

q Viral and retroviral antigens are frequently expressed in cancers such cervical carcinoma, and they also can be recognized by T-lymphocytes (16).

eluting peptide from MHC class I complexes

q The development of the new cloning technique SEREX with autologous serum (26) to identify tumor antigens based on spontaneous antibody responses in cancer patients made it possible to analyze tumor systems that typically do not grow in cell culture. Another advantage is that SEREX does not rely on tumor-specific T-cell lines (27)

http://www.licr.org/SEREX.html Ludwig institute for cancer research serex

differentiation antigens RAB38 (38) and NY-BR-1 (39),

38 = Serological cloning of a melanocyte rab guanosine 5'-triphosphate-binding protein and a chromosome condensation protein from a melanoma complementary DNA library. = 10910072 39 = 11280766

overexpressed antigens D52, NY-BR-62, and NY-BR-85. 17172736

q validation with mRNA expression patterns, Western blot, or ELISA, T-cell recognition (like "reverse immunology"),

q Based on the expression analysis, NY-BR-1 represents a new breast differentiation antigen.