E-mail+From+NEB+on+Dec+9th

Dear Kurt ,

Thank you for contacting NEB Technical Support. Your question and answer are below.

Oh actually it sounds like you may have exactly what I want! I want to express an scFv (single chain Fragment variable) protein as a fusion with the p3 M13 protein. An scFv gene is only about 750 bp and is like a mini antibody containing only the heavy chain variable region, light chain variable region, and a linker connecting the two. I think I can just insert my scFv gene sequence instead of a sequence for a peptide if I am not mistaken. Does your vector contain a SfiI site and NotI site that would allow me to clone my peptide or scFv sequence into the vector?
 * Your question:**

Could I get a link to your system(s) applicable for this? Thanks for any information you can offer me!

We have anecdotal evidence that large peptides may be displayed attached to pIII but we generally say 50 amino acids is the max. We do know some peptides/fragments display better than others so your 750 bp fragment is worth a shot. Unfortunately, to keep the pIII leader sequence intact, we did not design multiple restriction sites at the pIII insertion point. You may be able engineer a couple others in there but we exclusively use KpnI and EagI for cloning. The phage display vector is called M13KE and the RF DNA is available in #E8101S PhD Phage Display Cloning System,
 * Our answer:**

@http://www.neb.com/nebecomm/products/productE8101.asp

Regards,

Beth