Primer+order+for+random+hexamer+library+8-28-12

Since there could be stop codons in the 5' UTR region of full length mRNAs, we will make the human tumor cDNA library using random hexamer primers. The beginning of these random hexamer primers will contain the In-Fusion site necessary for ligating the genes.

pSMART2IFD in-fusion site information left side of in-fusion CGGGGTACGATGAGA right side of in-fusion TTGATACCACTGCTT reverse complement of right side of in-fusion AAGCAGTGGTATCAA

original thoughts on ordering 8-28-12 random hexamer primers for human tumor cDNA library