Compatibility+of+PCANTAB5E+and+M13cp-CT

see Two Plasmids One E coli for more information

__Compatibility of My Origins of Replication__ PCANTAB5E: F1 origin of replication M13cp-CT: based on M13mp19 plasmid which has the F1 origin of replication -If both plasmids truly do have the F1 origin of replication, then they are not compatible. -I suppose I could order primers and use PCR to check for sure. -If it does have the F1 origin of replication, I would need to replace it with a different origin of replication somehow. Compatibility table: [] >F1 Sequence gcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgatttgggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaa Length: 309 To check if my M13cp-CT and M13cp plasmids really do have this sequence I will need to do a colony PCR. The Primer3 website recommends these primers Primer_1_F F1_F ctcctttcgctttcttccct Primer_1_R F1_R aagggcgaaaaaccgtctat Link to order form for these primers: [] Link to receipt for these primers: []
 * Start: 70 || Length: 20 || Tm: 59.955 || GC%: 50.000 || ANY: 2.00 || SELF: 2.00 ||
 * Start: 253 || Length: 20 || Tm: 59.966 || GC%: 45.000 || ANY: 3.00 || SELF: 3.00 ||
 * Product Size: 183 || Pair Any: 6.00 || Pair End: 1.00 ||