amplify+specific+transcripts+from+RNA+4-11-13

I plan to try to amplify transcripts with RRS8+FRS8 and REATr+FEATr starting from RNA again.

With PrimeSTAR Max perhaps a reasonable procedure would be to add 1 uL of 2 uM each primer and do 30 cycles. Then add 1 uL 10 uM each primer, 5 uL H20, 5 uL PrimeSTAR Max Premix, and perform 10 more cycles. see