Primer-Blast+results+for+slainF+and+slainR

Results from Primer-Blast' Primer pair 1 Sequence (5'->3') Template strand Length Start Stop Tm GC% Self complementarity Self 3' complementarity Forward primer CCTGCCCTTAATAGGTTTTCTCC Plus 23 1012 1034 58.79 47.83 4.00 0.00 Reverse primer CTTTCCAACTGTCATCTTTCATGC Minus 24 1416 1393 58.84 41.67 4.00 2.00 Product length 405 Products on potentially unintended templates >NM_001113423.1 Mus musculus SLAIN motif family, member 2 (Slain2), transcript variant 2, mRNA

product length = 721 Forward primer 1 CCTGCCCTTAATAGGTTTTCTCC 23 Template 1320 ....................... 1342

Reverse primer 1 CTTTCCAACTGTCATCTTTCATGC 24 Template 2040 ........................ 2017

>NM_153567.2 Mus musculus SLAIN motif family, member 2 (Slain2), transcript variant 1, mRNA

product length = 799 Forward primer 1 CCTGCCCTTAATAGGTTTTCTCC 23 Template 1320 ....................... 1342

Reverse primer 1 CTTTCCAACTGTCATCTTTCATGC 24 Template 2118 ........................ 2095