Thap_plus_Rbm+chimeric+transcript

Note that the rbm chimeric transcript is more appropriately called Rbm14_Exon1_Rbm4b_Exon2

Original document from Shen which has the sequence information for this chimeric transcript as well as the information for the Thap and Tmem chimeric transcript "F:\kurt\storage\CIM Research Folder\DR\2013\6-23-13\chim transcript\thap_plus_rbm\Mus CDS of THAP and Rbm.doc"

rbm_fus GACAAGAGCCTACCACGTCAC 21bp 56.2 Tm

Rmb_fus and forward sequencing primer for pSMART2IF resulting sequence 6-23-13d1836 "F:\kurt\storage\CIM Research Folder\DR\2013\6-23-13\chim transcript\thap_plus_rbm\psmart2IF with rbm_fus and forward screen.SPF"

Rmb_fus and reverse sequencing primer for pSMART2IF resulting sequence 6-23-13d1839 "F:\kurt\storage\CIM Research Folder\DR\2013\6-23-13\chim transcript\thap_plus_rbm\psmart2IF with rbm_fus and reverse screen.SPF"

rbmF CAATGTCGATGGGGCGGATA 20 bp 53.7 Tm

rbmR CCACACCGATAGCAACCACT 20bp 53.7 Tm

primers last located at 4-26-13 -20 C chimeric transcript box I also have some aliquots here: 7-2-13d1700 -20 C box

sequence from rbmF and rbmR "F:\kurt\storage\CIM Research Folder\DR\2013\6-23-13\chim transcript\thap_plus_rbm\rbmF and rbmR.SPF"

possible primer pairs from primer blast "F:\kurt\storage\CIM Research Folder\DR\2013\6-23-13\chim transcript\thap_plus_rbm\possible primer pairs from primer blast.txt"

Primer order form "F:\kurt\storage\CIM Research Folder\DR\2013\6-23-13\chim transcript\Primer order form Kurt 6-23-13.xls"