Splenocyte+test+with+new+peptide+3-12-12

Splenocyte test with new peptide 3-12-12

I now have a new SIINFEKL peptide (part of the ovalbumin sequence). In the last ELISPOT I performed with Shen with the old peptide, the splenocytes with the target cells with no peptide exhibited more of a response than the splenocytes with target cells with peptide. I hypothesized that perhaps the peptide was harming my T cells somehow. Perhaps the DMSO used to dissolve the peptide was harming the cells. I plan to obtain some splenocytes, perform a trypan blue cell count, incubate the cells with peptide, and perform another trypan blue cell count. If it appears that the peptide is not killing my cells, I will perform another ELISPOT assay.

How should I dilute my new SIINFEKL peptide (H20, PBS, DMSO . . .?)? Research about dilution of SIINFEKL peptide 3-12-12

Protocols I plan to follow cardiac puncture Surgical Removal of Spleens Splenocyte Preparation Protocol

Results

Summary