In-Fusion+SMARTer+cDNA+Library+Manual

and here "C:\kurt\storage\CIM Research Folder\DR\2013\3-13-13\In-Fusion SMARTer cDNA.pdf"

Sequencing primers **Forward Screening Primer** (10 μM) TCACACAGGAAACAGCTATGA Tm of 50.3 according to oligomaster spreadsheet

**Reverse Screening Primer** (10 μM) CCTCTTCGCTATTACGCCAGC Tm of 56.2 according to oligomaster spreadsheet

The M13 forward and reverse primers can also be used M13 Forward Sequencing Primer (Sense Strand): 5'-GTAAAACGACGGCCAGT-3'

M13 Reverse Sequencing Primer (Antisense Strand): 5'-AGCGGATAACAATTTCACACAGGA-3'

SMARTer IIA Oligonucleotide Sequence as determined by sequencing AAGCAGTGGTATCAACGCAGAGTACATGGG

-- Other information

pSMART2IF Transcript orientation question 6-23-13