Dialysis+after+antibody+purification+with+Bart+2-28-12


 * Dialyze sample in Slide-A-Lyzer into 2 L 0.75 M NaCl in 10 mM sodium phosphate pH about 7.4 for 1 hr or several hours (use a 4 L beaker)
 * the 10 mM sodium phosphate can be made from a 200 mM sodium phosphate stock
 * 200 mM sodium phosphate stock can be prepared by diluting 28.39 g Na2HPO4 into 1 L. This can be adjusted to pH 7 (the pH may gradually increase over time as the solution ages) with 1 M HCl (required about 10 mL of this last time I adjusted)
 * Add 1 L 10 mM sodium phosphate to the dialysis beaker (now 0.5 M NaCl) for 1 hr or overnight
 * dialyze into 0.25 M phosphate
 * dialyze into 0.125 M phosphate
 * dialyze into 10 mM phosphate or just PBS
 * Concentrate using BSA if necessary
 * dialyze into 10 mM phosphate or just PBS