Identification+of+tumour+antigens+by+serological+analysis+of+cDNA+expression+cloning.+paper

Identification of tumour antigens by serological analysis of cDNA expression cloning. paper

Li G, Miles A, Line A, Rees RC.

Cancer Immunol Immunother. 2004 Mar;53(3):139-43. Epub 2004 Jan 13. Review. 2004

School of Science, The Nottingham Trent University, Clifton Lane, NG11 8NS, Nottingham, UK.

article is accessible "C:\kurt\storage\CIM Research Folder\DR\2013\1-26-13\some_serex_papers\Identification of tumour antigens by serological analysis of cDNA expression cloning..pdf"

Notes Identification of tumour antigens by serological analysis of cDNA expression cloning Cancer Immunology Immunotherapy 2004 Greece

q The immunoscreening of cDNA expression libraries constructed from human tumour tissues with antibodies in sera from cancer patents (SEREX: serological identification of antigens by recombinant expression cloning) provides a powerful approach to identify immunogenic tumour antigens

they start the paper off by talking about CTL, MHC, and peptides

q It would be surprising if cancer antigens induced only a cellular response and no antibodies. Furthermore, the development of high-titre IgG requires CD4 T-cell help.

SEREX a novel strategy using the antibody repertoire of cancer patients for the molecular definition of antigens was developed by Pfreundschuh and his colleagues Sahin and Tu¨ reci [15] Human neoplasms elicit multiple specific immune responses in the autologous host. paper

Their description of SEREX uses phage to present the tumor peptides

table 1 has the antigen categories identified

q antigen overexpression can lead to immunogenicity as it does in the case of HER-2/neu [36].

q aetiologically relevant gene products

^?definition of this? of or relating to the physical study of causation

q We have developed an array technique which can be used to rapidly analyse humoral response of multiple SEREX antigens using allogeneic sera.

q antibodies in human sera that react with bacterial or phage components can be eliminated by preabsorption of the sera with E. coli–phage lysate

they preabsorb antibodies against IgG as well q eukaryotic expression systems for SEREX should be developed