Test+of+FACS+sorting+of+cells+into+single+colonies+4-4-12

Test of FACS sorting of cells into single colonies 4-4-12

I will prepare an overnight culture of my top binding tumor cDNA library spot and meet with Tong Fu to see if the FACS works tomorrow. I will choose the top binding tumor cDNA library spot as shown in this presentation: http://www.azim58.wikispaces.net/file/view/Cancer+Meeting+3-28-12.ppt

The top spot is 2-17-12_RP_16C2 I will start an overnight culture of this.

After the FACS is performed, I plan to grow the cultures up and plate the isolated facs colony cultures as a well as a non-facs culture as a negative control. I will then pick several colonies and perform pcr to see if they have different sized inserts or the same insert.