Slain2+chimeric+transcript

original document from shen with the sequence that also shows where the chimeric transcript is "F:\kurt\storage\CIM Research Folder\DR\2013\6-23-13\chim transcript\Slain2\mus Slain2.doc"

Primer for Slain2 at the fusion site slain_fus

21 bp OligoMaster Tm: 54.2

primers last seen at 4-26-13 -20 C chimeric transcript box I also have some aliquots here: 7-2-13d1700 -20 C box

slainF CCTGCCCTTAATAGGTTTTCTCC 23bp 55.2 Tm

slainR CTTTCCAACTGTCATCTTTCATGC 24bp 53.9 Tm

products from slain primer pairs -sequence PCR amplified by slainF and slainR --Primer-Blast results for slainF and slainR -sequence generated from slain fus and pSMART2IF forward screening primer -sequence generated from slain_fus and reverse pSMART2IF primer -pSMART2IF_for plus slain_fus -pSMART2IF_for plus slainR -slainF plus slain_fus

slain2 chimeric transcript in pSMART2IF vector

Primer order form "F:\kurt\storage\CIM Research Folder\DR\2013\6-23-13\chim transcript\Primer order form Kurt 6-23-13.xls"

list of some sequencing results for slain primers portal 7-18-13