Cancer+Meeting+Presentation+9-14-11

This presentation was about the PCANTAB and rCompTT vectors for the phage library, protein detection, printing protein lysate onto different slide types, and a mention of the T cell assay gemini project.

C:\kurt\storage\CIM Research Folder\kwhittem\Presentations\2011\9-14-11 Cancer Meeting Presentation

Notes for Improvement: When cloning scFv into PCANTAB
 * understand and read about i j ratios
 * just use 3X more insert not 6X more insert otherwise different inserts can ligate to eachother
 * use calf intestinal phosphotase instead of shrimp phosphotase (follow protocol or titrate to see how little I need or don't use it at all)
 * precipitate with tRNA instead of glycogen
 * put a stuffer into the PCANTAB so that I can separate cut from uncut plasmid
 * Constructing PCANTAB-pRSET plasmid