Verify+ligation+of+rcomptt+frag+10-26-12

Verify ligation of rcomptt frag 10-26-12

Chose primers "C:\kurt\storage\CIM Research Folder\DR\2012\10-26-12\rcompTT ver\pcomb primer pick 10-26-12.SPF"

forward primer SAPTFC AGCGCCCAATACGCAAACCG

reverse primer SRNotI CGATTTCGGCCTATTGGTTA

see Numbers associated with pcomb rcomp ligation 10-26-1210

If the rcomp fragment is not present then the pcr product should be 284+1677+732 = 2693 If the rcomp fragment is present then the pcr product should be 284+1300+732 = 2316