Valiery's+Slides

Valiery's Slides are slides with a thick surface.

Slide Surface Chemistry

__Prewash for slides before running and after printing__ Valiery typically washes with the following 95% TFA 5 min DMF wash 95% Ethanol wash H20 wash Dry 1500 rpm 5 min

Note that on 8/26/11 I tried using Valiery's slides by just using the standard pre-wash buffer used in the lab. I thought that since I am using cell lysate, 95% TFA may be too harsh. I'll have to see how this turns out.

Comments about Slides -Valiery uses the same blocking and incubation buffer as usual with TBS instead of PBS. He thinks PBS might work okay, but said that TBS can slightly reduce the background. -Valiery says that the slides will have high background with sera. He recommended using a lower dilution of sera or blocking with sera first.