Printing+Tumor+cDNA+Library

042811 Printing cDNA Library onto Array

Cell Lysate Test Prints

[|Initial Notes (wuala link)] Initial Notes 042811 Printing Actual Library 010912 Printing 2-17-12_16E3 Spot

Planning -> 042811 -> 050211 -> (Results) [|5-16-11]

Planning ->[|062411] (2nd Library on Array Experiment) Protocols Standard Bacteria Lysis Protocol of Lab (from Alan): []

Diagram of Protocol for Plate Format Version 1: 4-28-11 Version 2: 5-24-11 Version 3: 6-20-11 (this version uses the correct amount of lysozyme) Slides Slides Types

Map of Experiments -> 5-3-11 Transform Library and aliquot into plate for overnight culture -> Perform colony PCR to see if diversity is maintained 5-4-11 Transformation efficiencies 5-5-11 Plating of overnight plate cultures 5-6-11 Colony PCR of Tumor Library Culture 5-9-11 Gel of colony PCR 5-10-11 Extract Tumor Library Proteins 5-13-11 Initial Tumor Library Array Experiment 5-16-11 [|Results of Initial Tumor Library Array Experiment]

5-17-11 Western Blot to Check for SMC1fs Protein 5-18-11 2nd Half of Western Blot 5-19-11 Western Blot Results (SMC1fs Protein Membrane) (with DH10B cells)

C:\kurt\storage\CIM Research Folder\kwhittem\Records in CIM Folder\Dated Records\2011\5-19-11

5-24-11 Extract proteins from BL21 containing pET32b and SMC1fs (see notebook) 5-31-11 Western Blot Results (SMC1fs Protein Membrane) (with BL21 cells)

C:\kurt\storage\CIM Research Folder\kwhittem\Records in CIM Folder\Dated Records\2011\5-31-11\SMC1fs Western Blot

6-2-11 Protein Extraction from BL21 cells (no plasmid, pET32b, and pGEX-SMC1fs-27mer) 6-3-11 Western Blot to Detect Extracted Protein 6-6-11 Western Blot Results

C:\kurt\storage\CIM Research Folder\kwhittem\Records in CIM Folder\Dated Records\2011\6-6-11\Western Blot and Coomassie Results

6-(8-9)-11 Extraction of Protein with lysozyme and sonication

6-13-11 Coomassie to Identify 14 kD band

"C:\kurt\storage\CIM Research Folder\kwhittem\Records in CIM Folder\Dated Records\2011\6-13-11\Coomassie Annotated 6-13-11.svg"

6-(14-16)-11 Test of anti-rabbit for 14 kD Band Autofluorescence measured here

"C:\kurt\storage\CIM Research Folder\kwhittem\Records in CIM Folder\Dated Records\2011\6-14-11\Scan for 14 kd autofluorescence\Scan for 14 kD autofluorescence annotated.svg"

test for secondary binding here C:\kurt\storage\CIM Research Folder\kwhittem\Records in CIM Folder\Dated Records\2011\6-17-11\Test of Secondary for 14 kD Band

6-20-11 Protein Expression w/less lysozyme 6-(21-22)-11 Western Blot to Check for 14 kD Band

"C:\kurt\storage\CIM Research Folder\kwhittem\Records in CIM Folder\Dated Records\2011\6-23-11\Western Blot for 14 kD Band 6-23-11\Annotated Files\Western Blot for 14 kD Band 6-23-11.svg"

6-(24-(7-5))-11 [|Printing cDNA Library 2nd Attempt]

7-8-11 Printing cDNA Library 3rd Attempt 7-14-11 Cell Lysate Plate Preparation Made denatured plate too 7/12/11 Cell Lysate Slide Results 3 (CodeLink and Aminosilane) 7/18/11 Cell Lysate Printing 4 (Non-denatured and denatured) 8/4/11 Cell Lysate Printing 5 (hepes and glycerol test) 11/23/11 Cell lysate printing onto ArrayIt Slides 11-23-11