B16F10

Some Documents "C:\kurt\storage\CIM Research Folder\DR\2013\4-13-13\download\B16 model protocol-1.pdf"

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 * "C:\kurt\storage\CIM Research Folder\DR\2013\4-13-13\download\Growing B16 Melanoma.pdf"

ATCC Subculture Protocol for B16F10 http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=CRL-6475&Template=cellBiology

Protocol:

Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:10 is recommended Medium Renewal: Every 2 to 3 days

stock last seen in our lab near freezer space rack B Box 5 a6 (lot 322557 12/2/05) rack K Box 7 B1 (passage 12)