Numbers+associated+with+pcomb+rcomp+ligation+10-26-12

The unmodified pComb3xSSv2 vector has the following features. SfiI 275 NotI 1952

1952-275 = 1677 vector is 4973 bp vector with fragment removed is 4973-1677 = 3296 bp

If the ligation worked properly, then the rCompTT fragment should be 1.3 kb