Protocol+for+Printing+cDNA+Library+1-9-12

Electroporation Protocol __Titration, Dilution, and Separation__ Titrate the pooled culture to determine transformation efficiency. Place culture at 4 c. Calculate transformation efficiency Dilute culture at 4 c so that there is 1000 cfu/(200 uL) Aliquot samples into wells of 96 well plates for overnight culture For overnight culture, taping plates to floor of incubator does not seem to work that well. Wedging the plates into a box so that they don't move, and taping the box to the floor of the incubator seems to work better. Protein Production in Plate Format