PhD+Phage+Display+Cloning+System

PhD Phage Display Cloning System http://www.neb.com/nebecomm/products/productE8101.asp Manual http://www.neb.com/nebecomm/ManualFiles/manualE8101.pdf

M13KE gIII Cloning Vector Information for this vector can be found on http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/dna_sequences_maps.asp Map http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/maps/M13KE_map.pdf Sequence http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/sequences/GenBank/M13KE.gbk.txt Pure Sequence M13KE Sequence The M13KE vector contains Acc65I-KpnI and EagI at the insert site.

E-mail From NEB on Dec 9th E-mail to Kathy about this phage display kit on 1-3-12

How does the position of the peptide insert in the PhD Phage Display System compare with the position of the scFv in the PCANTAB5E plasmid? Has anyone else used the M13KE vector for an scFv phage antibody library? If we change the restriction sites of the vector or insert, how will we do this?


 * Some Note from reading phage display cloning manual**