Thermocycle+Conditions+5-21-13

Thermocycle Conditions 5-21-13 has been associated with hs tdwn dyn (hot start touchdown dynazyme) protocol

95 C 30 s, 95 C hold, 95 C 2 min, 95 C hold, 95 C 2 min, 95 C 30 s, 65 C 30 s, 72 C 120 s, 95 C 30 s, 64 C 30 s, 72 C 120 s,  95 C 30 s, 63 C 30 s, 72 C 120 s,  95 C 30 s, 62 C 30 s, 72 C 120 s,  95 C 30 s, 61 C 30 s, 72 C 120 s,  95 C 30 s, 60 C 30 s, 72 C 120 s,  95 C 30 s, 59 C 30 s, 72 C 120 s,  95 C 30 s, 58 C 30 s, 72 C 120 s,  95 C 30 s, 57 C 30 s, 72 C 120 s,  95 C 30 s, 56 C 30 s, 72 C 120 s,  (95 C 30 s, 55 C 30 s, 72 C 120 s)X40, 72 C 5 m, 4 C hold

1st part to warm up thermocycler 2nd part to denature 3rd part to add polymerase and denature further 10 decreasing annealing temperature cycles for touchdown amplification for 40 cycles