Flow+Cytometry+CTL+Protocol

Overall flow cytometry CTL assay diagram

Flow Cytometry CTL Protocol
 * Mouse Immunization
 * Surgical Removal of Spleens
 * Splenocyte Preparation Protocol
 * Induction of Cytolytic Activity in CTL Precursors
 * Purification of T cells with Robosep Kit
 * Killing Assay
 * Killing Cells with Camptothecin for Positive Control
 * Caspase Kit
 * Annexin V protocol
 * Annexin V and PI or 7-AAD protocol
 * 7-AAD or PI protocol
 * Flow Cytometry

Note that I may want to: make shortcut sheet of calculations for CTL assay

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