how+to+make+a+gi+list

Why would you make a gi list? To perform a blast against only sequences from a certain species or custom collection of sequences.

How to make a gi list? Search for the sequences you want at the NIH protein page here: http://www.ncbi.nlm.nih.gov/protein To make a human species gi list search for human[organism] Then click the arrow next to send to->file->format->gi list->create file

Here's an e-mail from the blast help desk https://mail.google.com/mail/u/0/?ui=2&shva=1#search/Matten/13baa98324947c03

Hello,

See the manual for the "gilist" command. You supply a file of gi numbers corresponding to the sequences you want. Create that file by searching the NCBI Protein database with the query, human[organism]) NOT gbdiv pat[properties], then use the 'Send' menu to save that file in GI List format.

Best regards, Wayne