ovalbumin+in+immunoassays+3-22-12

search for people who have used ovalbumin in immunoassays http://www.nature.com.ezproxy1.lib.asu.edu/nri/journal/v4/n8/pdf/nri1413.pdf

In line with this result, two papers showed that

the primary in vivo CD8+ T-cell response to syngeneic

spleen cells loaded with ovalbumin requires CD4+ T-cell

help to be assayed by intracellular cytokine staining or

tetramer staining 7 days after immunization39,40.

39. Sun, J. C. & Bevan, M. J. Cutting edge: long-lived CD8 memory and protective immunity in the absence of CD40 expression on CD8 T cells. J. Immunol. 172, 3385–3389 (2004).

40. Le Bon, A. et al. Cross-priming of CD8+ T cells stimulated by virus-induced type I interferon. Nature Immunol. 4, 1009–1015 (2003).

These papers look like they inject e. coli produced ovalbumin into e coli, but they don't do an elispot with this whole protein.

here's some information about whole protein ELISPOTS


 * http://www.zellnet.com/elispotdiscussion/list.nhtml?profile=elispotdiscussion

> not available
 * forum response 3-22-12.1640
 * Dominant Ex Vivo Cross-Stimulation of CD8+ T-Cells With Whol ..
 * . In vitro IFN-γ //ELISPOT// responses to HIV Gag p55 //whole protein//
 * Optimization of A Dendritic Cell Based Whole Protein ELISPOT Assay for Immunological Monitoring

I'll just e-mail the company Worthington Biochemical > > Best regards, > Kurt Whittemore > > Graduate Student > Arizona State University > BIODESIGN INSTITUTE > Center for Innovations in Medicine > 1001 S McALLISTER AVE > TEMPE, AZ 85287 > > >
 * Is your "Ovalbumin, Purified" (LS003056) pure enough for immunoassays such as an ELISPOT? I have a friend who told me that pure protein has to be used. He had an experience in which he used protein produced from E. coli, and there appears to have been left-over E. coli components which greatly raised the immune response observed from the T cells in the ELISPOT. Thanks for any information you can offer me!