Transcript+orientation+question+6-23-13

An mRNA transcript can insert into the pSMART2IF vector in the forward orientation. However, the two in-fusion sites are identical. Therefore, sequences can insert into the vector in either orientation. When a sequence inserts into the vector in the reverse orientation, does it insert into the vector with the reverse sequence or the reverse complement sequence? <- The answer to this question it that the sequence inserts with the reverse complement sequence.

Inkscape file to address this question "F:\kurt\storage\CIM Research Folder\DR\2013\6-23-13\chim transcript\transcript orientation\transcript orientation question 6-23-13.svg"