How+to+get+good+results+with+lysate+on+nitrocellulose+slides

How to get good results with lysate on nitrocellulose slides
 * Perform overnight 23 C primary incubation
 * block with Super G buffer
 * use nitrocellulose slides the next day after the slide has been printed on rather than waiting too long
 * wash the pins between each print. (dip pins into peptide solution, pretap 5 times, print onto slide, wash pins, and then dip into next peptide solution)