Ideal+results+of+ds+cDNA+Synthesis

Basically a moderate strong smear from 0.5 to 5 kb should be detected.

A good overview of what a 2nd strand synthesis looks like can be found in the SMARTer cDNA library construction kit manual In-Fusion SMARTer Directional cDNA Library Construction Kit Here is the relevant page from the manual "C:\kurt\storage\CIM Research Folder\DR\2013\1-28-13\cDNA\Typical results of PCR and ds cDNA Synthesis.pdf"