Transformation+to+determine+efficiency+and+complexity+10-25-12

Transformation to determine efficiency and complexity 10-25-12

Finished ethanol precipitating the in-fusion reaction started by Mara.

Here are the sample IDs

10-24-12 MM Positive 18.1 ng/uL 1.61 260/280 Negative 20.8 ng/uL 1.85 260/280 Control A 30.4 ng/uL 1.68 260/280 Tumor A 40.0 ng/uL 1.67 260/280 Samples stored at Human tumor cDNA 8-3-12 -20 C

0.25 ng/(2 uL) = 0.125 ng/uL solutions were made from these to do a transformation in which there would be about 500 colonies on a plate spread with 50 uL solution if the transformation efficiency were 4e6 cfu/ug.