stem+cell+comments+from+John+1-24-13

original e-mail thread here https://mail.google.com/mail/u/0/?ui=2&shva=1#label/Career/13c6a04ac1c0d8ae

text from John

Yeah that's what I thought. This is a unipotent-lineage depletion set. So it would give you a population similar to CD34 positive selection. These cells will be 99%+ multipotent progenitors. (i.e. a cell with limited replicative potential that does not self-renew). Progenitors do not express the carcinoembryonic self-renewal associated genes that originally inspired your hypothesis. If you'd get rid of the progenitors and looked at only "true" (i.e. self-renewing, long-term repopulating) hematopoietic stem cells, you should see a big increase in signal if your hypotheses are true, and no increase in signal if they are wrong.

There are marker-sets that give you pure HSC in mice, but no easy magnetic things, due to the complicated marker combinations. So you'd need to use flow sorting (Meaning every single cell purified in this way will be sufficient for multilineage repopulation of an irradiated scid mouse). Unfortunately, no marker sets are known that enrich human HSC to more than 10%.