Second+Mouse+CTL+Experiment

Details of Dec-Jan 2012 Ova Immunization Record started 12-20-11

>> 4-6-12 Cardiac puncture and splenectomy
 * Tentative Schedule
 * Dec. 22nd and 23rd (Thurs and Fri): Genetic Immunization with 2 bullets per mouse with 1ug pCMVi-LS Ova
 * Is this 1 ug per bullet?
 * I couldn't find whether we used CpGs the first time or not
 * Jan. 5th: Protein Boost
 * Jan 19th: Obtain Spleen
 * [[image:Dec_2011.jpg]]
 * [[image:jan_2012.jpg]]
 * Actual Schedule
 * Genetic Immunization 12-22-11 (I think; Andrey and Felicia did this while I was gone)
 * Genetic Immunization 12-23-11 (I think; Andrey and Felicia did this while I was gone)
 * Protein boost 1-10-12
 * Protein boost 1-16-12
 * Protein boost and cheek bleed 3-30-12
 * 4-5-12 Cheek bleed
 * Details about Adjuvant and Protein
 * The adjuvant is currently suspended in PBS. I think there is about 700 uL left.
 * Saline solution of SAS stable up to 60 days. We suspended it on 10-13-11, and it expired on 12-12-11
 * Example resuspension of SAS: just add 1 mL PBS and then mix this 1:1 with antigen. Remaining solution can last 60 days.
 * 200 uL per mouse intraperitoneally
 * Example solution: 0.05 mg/mL protein of interest and 0.5 mg/mL SAS solution (all in PBS)
 * Recommended Antigen concentration range is 0.05-0.25 mg/mL saline
 * http://azim58.wikispaces.com/Sigma+Adjuvant+System

Mice from older cage:
 * Details about Mice
 * 5 female BALB/c mice, Protocol 1000 R
 * received 4 weeks old on 6-14-11
 * Current age as of 12-22-11: 6 months 8 days
 * Photo of Cage Card 2-9-12
 * Cage card ending on 4-6-12
 * [[file:Ova mouse end 1 4-6-12.pdf]]
 * [[file:Ova mouse end 2 4-6-12.pdf]]