Cut+to+test+for+ligation+10-15-12

Cut to test for ligation 10-15-12

Numbers associated with pcomb rcomp ligation 10-26-12

So I will be able to see if the ligation worked or not, and whether or not my plasmid is the way that I expect it is.

None of the pcomb rComp ligation plasmid samples even had any DNA on the gel! Anyway, regardless of these results, the next time I do a digest and ligation I will need to make sure that the uncut plasmid is well separated from the cut plasmid backbone.