Designing+primers+for+PCR+screen+11-7-12

For the genes found in 8-26-12 Plasmids for verification I would like to know which ones are present or not present in normal cells, general 4T1 cells, and my specific 4T1 cells. I would also like to know whether genes are novel genes or not.

I will start by looking at plasmid number "41" "C:\kurt\storage\CIM Research Folder\DR\2012\11-7-12\sequence analysis\Analysis of Plasmid 41 11-7-12.docx"