RAW+cells+taken+from+freezer+11-29-11

Cell Type: RAW 264.7 macrophages from BALB/c ascites transformed with Abelson murine leukemia virus complete DMEM should be used Don't use trypsin Subcultivation ratio: 1:3 to 1:6 is recommended Medium Renewal replace every 2 to 3 days

Original vial in freezer was from Carlos
 * RAW 264
 * Nov 2008
 * P5
 * located in Rack J Box 1 Position I 6-8

Note that Kary has also ordered some fresh RAW 264.7 which we could have used, but we did not.

We thawed the vial at 37 C, placed in ethanol. We then placed the cells in about 13 mL complete DMEM media. Note that the cells were centrifuged and resuspended to remove DMSO.

11-30-11 10 more mL complete DMEM was added.