2nd+immunization+calendar

Immunization Calendar for CTL Ova experiment Here is a tentative immunization calendar. "C:\kurt\storage\CIM Research Folder\DR\2013\1-21-13\data_download\2nd_immunization_calendar.PNG"

Actual immunization schedule 9/27/11 Immunized 3 mice 2 bullets with plasmid for Ova with 1 ug (1 bullet per outside ear) 9/29/11 Immunized 3 mice 2 bullets with plasmid for Ova with 1 ug (1 bullet per inside ear) 10/13/11 Performed protein boost with SAS adjuvant (200 uL solution per mouse. the solution consisted of 0.05 mg/mL of ovalbumin protein and 0.5 mg/mL SAS) 10/18/11 Obtained 1 naïve spleen and 1 immunized spleen 10/20/11 (2 Cheek bleeds. 1 splenoctomy & cardiac puncture.)? 12/6/11 1 splenoctomy of immunized mouse