Transfer+of+Lysate+to+384+well+plates+2-23-12

3000 lysate wells will be transferred to 384 well plates.

The basic process for this was to shake my 96 well plates in a plate mixer at 1250 rpm for 30 s, and then centrifuge 2000 pm 5 min. The Biomek protocol (something like 10 K Kurt was used to transfer the samples over to 384 well plates).

Spreadsheet of 3000 wells "C:\kurt\storage\CIM Research Folder\DR\2013\1-21-13\data_download\1014\4T1 Tumor Mouse cDNA Library 2-23-12.xls"

Spreadsheet of 3000 wells with 384 info "C:\kurt\storage\CIM Research Folder\DR\2013\1-21-13\data_download\1014\4T1 Tumor Mouse cDNA Library with 384 info 2-23-12-1.xls"

3K_lysate CSV file for making gal file

"C:\kurt\storage\CIM Research Folder\DR\2013\1-21-13\data_download\1014\3K_lysate.csv"

Original Gal file with "E"s irritatingly recognized as scientific notation

"C:\kurt\storage\CIM Research Folder\DR\2013\1-21-13\data_download\1014\3K_lysate_gal.gal"

Gal file with Es interpreted correctly

"C:\kurt\storage\CIM Research Folder\DR\2013\1-21-13\data_download\1014\3K_lysate_gal_correct_es.gal" 3K Lysate gal file with qcorrect esq 5-5-13

Gal file scaled for 5 um

"C:\kurt\storage\CIM Research Folder\DR\2013\1-21-13\data_download\1014\3K_lysate_gal_3-2-12.gps"

Cell lysate Gal file scaled for 10 um "L:\storage\CIM Research Folder\DR\2012\5-5-12\exple 3k lysate for 10 um.gps"

Regex to replace Es mistakenly identified in scientific notation find: (\d+).\d+E\+0?(\d+) replace: $1E$2

Shifted wells 2-24-12

Modified SMC1fs positive control wells by diluting them 1 to 1000 in PUC19 lysate

New Spreadsheet can be found here: "L:\storage\CIM Research Folder\DR\2012\5-2-12\4T1 Tumor Mouse cDNA Library with 384 info 5-2-12.xls"

ID of SMC1fs spots 2-17-12_SMC1fs_1A1 2-17-12_SMC1fs_16E9 1-8-12_SMC1fs_1B8

see arranging plate information for gal file creation