Map+of+Experiments+for+CTL+Flow+Cytometry+Assay


 * Gel of OVA plasmid 6-13-11
 * Initial 3T3 cell culture 6-20-11
 * Test of caspase kit 10-12-11
 * Protein boost with OVA 10-13-11
 * Flow cytometry 10-13-11
 * Caspase kit test (2nd time) 10-17-11


 * Fresh media for targets and effectors 10-23-11
 * CTL Assay 1: Killing Assay 10-24-11
 * T cell enrichment 10-26-11
 * Caspase Staining 10-26-11
 * Flow Cytometry to analyze cells
 * Ova Immunization ELISA 10-25-11
 * NIH 3T3 Apoptosis 11-2-11
 * Camptothecin Test 11-9-11
 * Cell Staining 11-10-11
 * T Cell purification 11-21-11
 * IL-2 and Peptide Addition 12-7-11
 * Killing Assay with Caspase 12-13-11
 * T cell assay maintenance
 * Killing Assay with Annexin V 12-21-11
 * Passaged 3T3 and CRL2116 cells 1-3-12
 * Preparation for T cell purification comparison 1-4-12
 * T Cell purification to compare old T cell enrichment kits 1-16-12 (for 1-5-12)
 * peptide preparation 1-23-12
 * peptide plate transfer 1-23-12.2216
 * Ova protein mouse immunization 1-26-12
 * test of decanting after fluor is added 2-3-12
 * ELISPOT w/Ova splenocytes 2-9-12
 * ELISPOT w/Ova splenocytes 2-11-12
 * Imaged ELISPOT plates 2-13-12
 * SIINFEKL Peptide Incubation 3-13-12
 * ELISPOT w/Ova Peptide 3-14-12
 * Ova protein boost 3-30-12
 * ova splenocyte test 4-6-12
 * killing assay 4-8-12
 * bone marrow surgery 6-7-12
 * Ova Bullet preparation 6-18-12
 * Ova Protein Boost 7-3-12
 * Ova ELISA 7-13-12 (Ova ELISA 7-13-12)
 * B16F10 Cell Culture 7-17-12
 * chromium release assay 8-17-12 to 8-18-12
 * CRA with more E:T ratios 8-21-12
 * SIINFEKL dilution 9-26-12
 * Ova protein boost 10-2-12
 * Ova CTL Assay 10-8-12
 * Ova mouse immunization 10-15-12
 * CRA parameter test 10-18-12
 * ova ctl assay w/o tissue culture flask 10-20-12
 * CRA Parameter Test 10-18-12
 * Ova CTL Assay w/o tissue culture flask 10-20-12
 * aged-young cr release assay 1-4-13
 * obtained splenocytes from injured mice 1-6-13
 * aged-young cr release assay 1-10-13