random+hexamer+primers+for+human+tumor+cDNA+library

Random Hexamer primers for tumor cDNA Library data sheet

"C:\kurt\storage\CIM Research Folder\DR\2012\8-31-12\Random hexamer primers 8-31-12.pdf"

Primers hexL -> new name: FSHX (for first strand hexamer) CGGGGTACGATGAGANNNNNN hexR -> new name IFF (for In-fusion forward) AAGCAGTGGTATCAANNNNNN InFL -> new name IFR (for In-fusion reverse) CGGGGTACGATGAGA InFR AAGCAGTGGTATCAA

100 uM stock stored at Human tumor cDNA 8-3-12 -20 C 10 uM working solution at 4 C box 082512 dot 1020

These random hexamer primers will be used in addition to poly T primers. I will use the same ratio of poly T primers to random hexamer primers as that found in a commercial random hexamer mix. In the random primer mix from neb (http://www.neb.com/nebecomm/products/products1330.asp) the mixture contains 35 uM hexamers and 25 uM poly T primers so the poly T primers make up 25/(35+25)*100 = 41.7% of the mixture. I'll use this ratio for the human tumor cDNA library.

see poly dT random hexamer primer mix

Notes on use of these primers 12-12-12