PrimeScript+Reverse+Transcriptase

from Takara

Product Slip and Protocol "F:\kurt\storage\CIM Research Folder\DR\2013\1-16-13\PrimeScript\PrimeScript Reverse Transcriptase Product Slip.pdf"

User Manual "F:\kurt\storage\CIM Research Folder\DR\2013\1-23-13\primescript\UTF-8'en-us'2680Q_DS.v1205Da_2.pdf"

What is the minimum amount of RNA that can be used in this reaction? In the In-Fusion SMARTer Directional cDNA Library Construction Kit they use 5-100 ng poly A+ RNA.

Note that this polymerase may not be ideal for use with poly T primers with only one or two base pairs to bind to the transcript like the primer in the In-Fusion SMARTer Directional cDNA Library Construction Kit for an example of some cDNA results obtained using those primers see received random hexamer tumor library clones 2-7-13