PCR+Screen+for+13+validated+mouse+chimeric+transcripts+5-2-13

Screened pools from tumor cDNA library for presence of 13 validated mouse chimeric transcripts from Hojoon. Mara started this PCR, and then I came in for the "hot start" and thermocycle part. experiment performed on 5-2-13

Original Gel Images found here S:\Research\Cancer_Eradication\Discovering tumor specific antigens\Tumor cDNA Library\5-2-13

Annotated Inkscape files here "F:\kurt\storage\CIM Research Folder\DR\2013\5-7-13\chim transc\chimeric transcript gels 5-2-13.svg" "F:\kurt\storage\CIM Research Folder\DR\2013\5-7-13\chim transc\chimeric transcript gels 2 5-2-13.svg"

Table indicating presence of transcripts in different samples "F:\kurt\storage\CIM Research Folder\DR\2013\5-7-13\chim transc\Chimeric transcript screen 5-2-13.xls" Note that 8 of the 13 validated mouse transcripts (from Hojoon) are present in at least one of the two tumor cDNA library plasmid pools that I screened. They originally only detected 4 of these transcripts in 4T1 cDNA previously.