Gene+Screening+Portal+3-30-12

SMC1fs PCR in 4T1 cDNA lysate pools 3-30-12 Test of FACS sorting of cells into single colonies 4-4-12 SMC1fs PCR in total 4T1 cDNA 5-2-12 Test of FACS sorting of cells with Cell Tracker Orange 5-8-12 Screen of increasing tumor spot 6-17-12 Need to Order Clontech Kit Sequencing Primer 7-13-12 Sent selected colony plasmids from 16E3 library for sequencing 8-13-12 Start planning to validate cDNA clones 1-7-13 validate cDNA clone work 2-1-13 Start process to construct transcript specific cDNA for blunt end cloning and sequencing amplify specific transcripts from RNA 3-18-13 amplify specific transcripts from RNA 3-25-13