General+Meeting+Presentation+7-25-12

This presentation was about the construction of the cDNA library and contains the pictures of the HiGro, and Biomek. The presentation also contains a few summary slides about the phage library and the pComb3xSS vector that will be used. Some antibody purification with peptides and the idea of using peptides at 4X density is also mentioned.

version with links to info "C:\kurt\storage\CIM Research Folder\kwhittem\Presentations\2012\General Meeting Presentation 7-25-12\version with links to info\General Meeting Presentation 7-25-12 (2).pptx" version without links to info "C:\kurt\storage\CIM Research Folder\kwhittem\Presentations\2012\General Meeting Presentation 7-25-12\version without info links\General Meeting Presentation 7-25-12.pptx"

My last general meeting presentation was in April (see General Meeting Presentation 4-1-12). At that time I presented my immunosignature entropy concept though. This time I will present my main thesis work.

For my presentation I should probably have about 20-25 slides of content. Here's the basic layout I think I want
 * Introduction
 * Slide outlining what I'll talk about for the rest of the presentation (screening tumor cDNA library, phage library, purifying antibodies)
 * Screening tumor cDNA library
 * a little background of construction
 * show SMC1fs titration results
 * show secondary antibody titration results
 * picking 1000 colony spot
 * results of single colony spot
 * plans
 * Phage library
 * a little background
 * plans for plasmid construction
 * Purifying antibodies
 * a little background
 * result of experiment
 * plan for higher density and orientation issues
 * Conclusion

I think I'll borrow some slides from the last general meeting powerpoint related to this presentation L:\storage\CIM Research Folder\kwhittem\Presentations\2011\5-25-11 General Meeting Presentation and also some slides from my last committee meeting L:\storage\CIM Research Folder\kwhittem\Presentations\2012\April 2012 events\presentation

I would like to make some diagrams explaining the orientation issue of the peptides on the beads. Diagram creation info: L:\storage\CIM Research Folder\DR\2012\7-23-12\peptide orientation diagram

Summary of General Meeting Presentation 7-25-12

Comments during presentation Chris said I could have checked the eluted antibody fraction on a silver stain or Western blot (Bart mentioned or dot blot) rather than putting it onto the array. This would have been cheaper.

Stephen thinks it could be better to have my human tumor cDNA library in the incorrect orientation and out of frame just like this mouse one is. I'm not so sure about this. . . He said he would talk to me later.

Stephen also mentioned that I may want to publish some papers just on the screening platform that I have set up. This could be a very good idea.