poly+dT+primer+portal+2-1-13

The purpose of a poly dT primer would be to amplify poly A mRNA. One might want to make 1st strand cDNA from RNA, and then 2nd strand cDNA from 1st strand cDNA.

Here are some primers I will order.

dT1 poly T primer for in-fusion with vector in directional SMARTer In-Fusion kit 5’–CGGGGTACGATGAGACACCATTTTTTTTTTTTTTTTTTTTVN–3’

dT2 2nd strand synthesis primer for in-fusion with vector in directional SMARTer In-Fusion kit 5’–CGGGGTACGATGAGACACCATTTTTTTTTTTTTTTTTTTT–3’

dT3 poly T primer (this could be used for 1st and 2nd strand synthesis 5'-TTTTTTTTTTTTTTTTTTTTVN–3’

"C:\kurt\storage\CIM Research Folder\DR\2013\2-1-13\poly dT primer\Primer order form Kurt 2-1-13.xls"