8-26-12+Plasmids+for+verification

082612 Plasmids for verification

Here is the key for the plasmids I would like to select to produce protein from


 * Well from 16E3 Library || Colony number (1-10) || Plasmid Number || ID Given to Plasmid ||
 * "21E11" || 4 || 4 || 082612_4 ||
 * 22H10 || 1 || 11 || 082612_11 ||
 * 21G4 || 1 || 21 || 082612_21 ||
 * 11G12 || 1,3 || 31,33 || 082612_31,082612_33 ||
 * 7F12 || 1,2 || 41,42 || 082612_41,082612_42 ||
 * "7E12" || 1 || 51 || 082612_51 ||
 * 10A5 || 1,2 || 61,62 || 082612_61,082612_62 ||
 * 13A5 || 1 || 71 || 082612_71 ||
 * 29A5 || 1 || 81 || 082612_81 ||

I will need to transform these plasmids into e coli. Pick a colony for overnight culture, and then produce the protein.

These plasmids are located here: Selected colony Plasmids from 16E3 library 8-13-12 KW at box in -20 C nearest to benches

see also Sent selected colony plasmids from 16E3 library for sequencing 8-13-12

11-19-12 Note a better table than the one found on this page (one with stop codon information and blast information) can be found here.

"C:\kurt\storage\CIM Research Folder\DR\2012\11-19-12\sequence analysis\8-26-12 plasmid details.xlsx" Sequencher file "C:\kurt\storage\CIM Research Folder\DR\2012\9-10-12\tumor lib sequences\Analysis of sequences for stop codons 2 9-10-12.SPF"

Ordered primers for validation 11-19-12

actually, see powerpoint here "C:\kurt\storage\CIM Research Folder\DR\2012\11-25-12\cDNA Sequence Analysis\cDNA Sequence Analysis with source info 2 11-25-12.pptx" also "C:\kurt\storage\CIM Research Folder\DR\2012\11-25-12\cDNA Sequence Analysis\cDNA sequence analysis clean 12-11-12.pptx" (other files such as annotated ape files found here) C:\kurt\storage\CIM Research Folder\DR\2012\11-25-12\cDNA Sequence Analysis

Information about some of the proteins in the final screen "C:\kurt\storage\CIM Research Folder\DR\2013\3-15-13\Information about some of the proteins in the final screen.docx"

versions of protein sequences from associated transcript sequences

9-10-12 I did some earlier analysis on 9-10-12, but I was not able to reproduce all of the BLAST results with this analysis. For example I could not reproduce the DEAD box match Analysis of sequences for stop codons 9-10-12 "C:\kurt\storage\CIM Research Folder\DR\2012\9-10-12\tumor lib sequences\Analysis of sequences for stop codons 9-10-12.docx" "C:\kurt\storage\CIM Research Folder\DR\2012\9-10-12\tumor lib sequences\Analysis of sequences for stop codons 9-10-12.xlsx"