Taq+PCR+Protocol

manual

"F:\kurt\storage\CIM Research Folder\DR\2013\5-16-13\Taq Polymerase.pdf"

http://biotech.wku.edu/online_manual/Taq%20Polymerase.pdf

TaqMaster buffer can also be used TaqMaster is a buffer additive that improves thermostability (enzyme half-life) and processivity of Taq DNA polymerase by stabilizing the enzyme during PCR.TaqMaster makes the enzyme less sensitive to exogenous PCR-inhibiting contaminants. TaqMaster is chemically stable, inert, and does not change the optimal primer annealing temperature or the melting behavior of template DNA. "F:\kurt\storage\CIM Research Folder\DR\2013\5-16-13\mastertaq kit manual_5prime_1044367_032007.pdf"

TaqMaster buffer should be heated at 65 C and vortexed vigorously until completely dissolved before use and before storage.