Text+for+indexing+8-16-12+dot+1146

CSGNRVWWYTKQ. . peptide removed Images of Membranes Tumor Naive No Antibody Minimum Displayed Value: 200 Maximum Displayed Value: 6000 Note: Both the naive and tumor samples had about the same intensity for some of the 6 random peptides. Both types of sera displayed high intensities for the NRVWW, YNPIW, and VNGEY peptides. The VNGEY peptide was the only peptide for which the tumor sera had higher intensity than the naive sera. Both sera types had a low intensity for the negative control SIINFEKL peptide. One thing to keep in mind is that the tumor sera was essentially diluted 1 million fold (1000 fold dilution during the antibody purification since the peptide was eluted in a 1 mL fraction and 1000 fold for the dot blot assay). The naive sera was only diluted 1000 fold for the dot blot assay. Therefore, the two sera types are not directly comparable. If I want a better comparison, I could redo the dot blot assay by diluting the naive sera 1 million fold or even purify the naive sera using the same random peptide tentagel bead method, and then perform the dot blot. Dot Blot Summary 8-16-12 Tumor sera was purified using a mixture of Tentagel beads with 6 random peptides. The 1st 1 mL elution fraction was then diluted 1 to 1000 for a dot blot. Naive sera was used directly by diluting 1 to 1000 for a dot blot without the antibody purification. A membrane with no applied antibody (no primary or secondary) was also scanned on the Typhoon. Any AF647 direct labelled secondary antibodies were detected, and the results are summarized below.