Some+Note+from+reading+phage+display+cloning+manual

linkage between genetic material and protein or peptide on outside of phage

If the displayed peptide is sufficiently short (< 50 residues), the infectivity function of pIII is not affected, and all 5 copies can carry displayed peptides without measurable attenuation of phage infectiv- ity (6)

M13KE derived from M13mp19

New England Biolabs currently offers 3 pre-made random peptide libraries, as well as the cloning vector M13KE for construction of custom libraries.

bioactive peptides cell targeting peptides

M13 is not a lytic phage

cloning sites have been introduced at the 5 ́ end of gene III for display of short peptide sequences as N-terminal pIII fusions

M13KE is a phage rather than a phagemid vector. All 5 copies of pIII will be fused to the cloned peptide. The plasmid has no antibiotic resistance. Also no helper phage is required.

Quote: "We maintain an up-to-date list of applications of the Ph.D. kits in the technical reference area of neb.com"

TAG stop codons are suppressed by glutamine in ER2738 (glnV)