Ordered+primers+for+validation+11-19-12

Ordered primers for validation 11-19-1

Primer order form "C:\kurt\storage\CIM Research Folder\DR\2012\11-19-12\sequence analysis\Kurt Primer Order Form 11-19-12.xls"

received primers 11-26-12 stock of primers last seen Tumor Library 11-29-12 KW -20 C Box 10 uM primers last seen in 4 C Kurt 11-16-12

The location of the primers along the transcript can be found in the ape files in the subfolders here C:\kurt\storage\CIM Research Folder\DR\2012\11-25-12\cDNA Sequence Analysis Basically the primers amplified sequence from the beginning of the sequence in the bacteria clone to the end of the sequence. In longer transcripts which contained a stop codon in the bacteria before the end of the transcript sequence another reverse primer was used to amplify a shorter sequence as well.

KW4F CGCAGAGTACATGGGAAGGATC KW4R CAGATAGAACAGCAGACACCTTTATTAC KW11F CGCAGAGTAGGTTGTGTTAAGTC KW11R ACCAGAGATACAATGAACATATTAAAAAG KW21F CGCAGAGTACATGGGATGG KW21R ACGCTTGTGTGGCATGATTGG KW21RS TCAAAACCATTCTACCTCATTAGC KW31F CGCAGAGTACATGGGGTACANG KW31R CACCATTATTTACAATAAAGTAAATATACAGTTGG KW41F AGTATCCATTGAAAATTTATTATGCATTAG KW41R CGCAGAGTACATGGGATTCC KW42F GGGCAAGTACACGCGATCATAG KW42R ATAAAGCAATTCCAAAGTTTATTAGCC KW51F CGCAGAGTACATGGGGGAG KW51R GACAATGCCCTAATATGTCTGTGTCC KW51RS AATTCTTTGGGGCAAGACACTACATAG