T+Cell+Purification+to+Compare+Old+T+Cell+Enrichment+Kits+with+New+1-4-12

T Cell Purification to Compare Old T Cell Enrichment Kits with New 1-4-12 (files stored at 1-5-12)

Robosep Kit Used: Negative Selection Mouse T Cell Enrichment Kit 19751 Lot 11F39752 Antibodies used for Flow Cytometry: Product Information Sheets for Antibodies for Detecting T Cells 12-14-11

Protocols Followed: Splenocyte Preparation Protocol Purification of T cells with Robosep Kit Antibody Staining Protocol. The product sheets for the antibodies don't give very much information, but I would imagine the protocol would be very similar to the Annexin-V antibody Annexin V and PI or 7-AAD protocol Flow Cytometry
 * Some flow cytometry settings
 * FS, Volts 635, Gain 5.0
 * SS, Volts 431, Gain 50.0