Overnight+primarysecondary+only+Tecan+Protocol

Protocol wash TBST 30 s hybridization (for blocking buffer) 23 C 1 hr Wash TBST 30 S hybridization (primary) 23 C 16 hr Wash TBST 30 s hybridization 23 C 1 hr Wash TBST 30 s Wash H20 30 s slide drying 30 C 5 min

Image of protocol from Tecan computer monitor "C:\kurt\storage\CIM Research Folder\DR\2013\3-12-13\overnight primary secondary only.jpg"