RT-PCR+of+transcripts+figure+3-10-13

This figure will show whether transcript sequences could be amplified from various RNA sources using certain primers.

A version of the figure was present in the presentation here C:\kurt\storage\CIM Research Folder\kwhittem\Presentations\2012\Fall 2012 Committee Meeting Presentation 12-19-12 svg file here C:\kurt\storage\CIM Research Folder\DR\2012\12-18-12\pcr analysis\pcr analysis 12-18-12.svg

New Figure with Different labels here

"C:\kurt\storage\CIM Research Folder\DR\2013\3-10-13\work on paper\RT-PCR Figure\RT-PCR Figure.svg"

What exactly is this experiment about? Info about experiment here: Initial PCR Screen 11-26-12 The location of the primers along the transcript can be found in the ape files in the subfolders here C:\kurt\storage\CIM Research Folder\DR\2012\11-25-12\cDNA Sequence Analysis Note that all of the RNA samples all contain the expected bands for the fragments amplified with this set of primers. However, the normal tissue also contains many other bands for the Wfdc17_S primer set which the tumor samples do not contain. This may be due to the tissue type difference since the tumor samples are breast tissue and the normal skin tissue is from the ear of a mouse.

Printout of Figure 4 here "C:\kurt\storage\CIM Research Folder\DR\2013\3-10-13\work on paper\RT-PCR Figure\Figure 4.pdf" -

^ Note that I actually think I don't want to include this figure in the paper. All these PCRs show is that the transcripts were in the RNA pool as expected. There is only one sequence (Wfdc17_S) which shows something different between the RNA samples. However, the forward primer for this sample bound to part of the SMARTerIIA oligo sequence. The primer was designed this way before I realized that the SMARTerIIA oligo sequence existed and that this sequence was not part of the transcript sequence. Therefore, I'm not sure if much can be included or if much information can be gained from this figure.