Designing+Random+Pentadecamers+2-13-13

Schematic "F:\kurt\storage\CIM Research Folder\DR\2013\2-13-13\random pentadecamer\cDNA library random pentadecamer plan 2-13-13.svg"

Sequencher alignment of primers "C:\kurt\storage\CIM Research Folder\DR\2013\2-13-13\random pentadecamer\pentadecamer primers 2-13-13.SPF"

Here is the random pentadecamer primer order form "C:\kurt\storage\CIM Research Folder\DR\2013\2-13-13\random pentadecamer\Kurt primer order form 2-13-13.xls"

Here are the primers on the form IFF15R (In-fusion forward 15 bp random) = AAGCAGTGGTATCAANNNNNNNNNNNNNNN IFR15R (In-fusion reverse 15 bp random) = CGGGGTACGATGAGANNNNNNNNNNNNNNN 15R (15bp Random) = (N)15 = NNNNNNNNNNNNNNN

primers located in 4 C Kurt 11-16-12 (10 uM) and random hexamer library 2-5-13 KW -20 C box (100 uM)

Here are some calculations about how many possible 15mers 1 uL of 10 uM primer solution would contain. "C:\kurt\storage\CIM Research Folder\DR\2013\2-13-13\random pentadecamer\random pentadecamer calculations.jpg"

2-18-13 received primers "C:\kurt\storage\CIM Research Folder\DR\2013\2-18-13\15R primer order sheet\15R primer order sheet.pdf"