questions+about+ordering+random+nucleotide+oligos

2-13-13 contacted SIGMA about ordering random oligos my message Is it more expensive to order oligos with random nucleotides as compared with oligos with non-random nucleotides? How large would the price difference be? Also, someone told me that in the past it was not possible to order oligos with a random nucleotide at the 3' end of the oligo? Is this still the case or can I order an oligo with a random nucleotide at any position? I am asking these questions because I think I will soon order random pentadecamers (15 bp oligos) to help me construct a cDNA library. Thanks for any help and information you have to offer me!

--- message back from SIGMA

Good Afternoon Kurt,

You can order a degenerative base at any position in you sequence. There is no premium in pricing for the degenerative bases unless you would like a unique mixture. You should be able to place the order online with no issues. The price for a desalted oligo should cost about $4.80. If you have any questions or concerns please let me know.

Regards,

--- see message here https://mail.google.com/mail/u/0/?ui=2&shva=1#label/Career/13cd4d76e7c79666