IgG+Purification+with+Protein+G

Protein G Agarose from Thermo Scientific (there are several different versions of this kit) 20397, 20398, 20399, 22851, 22852

Original Kit Instructions http://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=1&ved=0CDYQFjAA&url=http%3A%2F%2Fwww.piercenet.com%2Finstructions%2F2160576.pdf&ei=zOIyT6z9CcnciQKZ-KmHCg&usg=AFQjCNHB1cJCcac0kSk72_k0yZGxgdWyMg&sig2=hcLMxdeqfhpqI_ngOrLS8w

Modifications or Additional Notes to Protocol
 * It may be better to incubate sera/binding buffer solution in column with protein G solution while the column is sealed and shaking on a rocker for a time
 * Note that when nanodropping, make sure to blank with a solution containing the elution buffer and neutralization buffer in the appropriate ratio
 * Binding Buffer: 0.1 M Sodium Acetate pH 5.0 (pH adjusted with 1 M HCl)
 * Elution Buffer: 0.1 M citric Acid pH 2-3