Shrimp+Alkaline+Phosphatase+Promega

Link to information sheet: http://www.oardc.ohio-state.edu/tomato/604.03/Module1/promega%20shrimp%20alkaline%20phosphatase.pdf

Promega SAP 1 u/uL (500 u in tube total) Cat # M820A or 25856303. Should be stored at -20 C

SAP can inactivated at 65 C 15 min, whereas CAP (Calf Alkaline Phosphotase) cannot be.

Main Points from Protocol
 * 10 units SAP/μg vector in a final volume of 30–50μl. Stock is 1 unit/uL
 * Incubate at 37°C for 15 minutes.
 * Incubate at 65 C for 15 min

SAP Protocol