Genetic+Immunization+Bullet+Preparation

the protocol of these files can be found on the shared drive "S:\Research\GeneVac\Protocols\Standard Operating Procedure for Making Bullets - CaCl2 revised_9-26-2011.doc" "S:\Research\GeneVac\Protocols\nanogold experiment design-updated june6,2012.xls"

Protocol >
 * Take the calculated aliquot of colloid gold and transfer it into a fresh plastic tube.
 * Add the calculated aliquot of DNA to the colloid gold
 * Mix. The color of the solution should change to more violet tone. Leave at RT for 5 min. Aggregate particles often start to form
 * Add the calculated aliquot of charged micro gold to the mix.
 * Mix occasionally at RT for 10min. Gold sedimentation rate should increase and supernatant discolorate. Mix at about 3 min intervals
 * Briefly spin for 1 min 1000 rpm gold by centrifugation
 * Aspirate the supernatant
 * Resuspend the gold pellet in the residual liquid and add 1ml of ethanol to the slurry while vortexing
 * Briefly spin gold by centrifugation
 * Aspirate the supernatant
 * Briefly spin gold again and aspirate the residual ethanol
 * Resuspend the gold pellet in the calculated amount of butanol and transfer into Teflon tubing
 * Let the gold settle in the tubing for 5-10 min
 * Drain the butanol and dry the gold by flowing N or Ar gas gently through the tubing
 * Cut tubing into bullets with tube cutting device (1/2" pieces)
 * Store over dryrite.

Here's an example protocol Felicia and I used on 6-18-12 "F:\kurt\storage\CIM Research Folder\DR\2013\5-27-13\wiki_download\bullet preparation protocol 2 6-18-12.pdf"