Mouse+Immunization


 * Mouse Immunization**


 * Good adjuvant for getting a T cell response: Incomplete Freunds
 * Source: Blattmann
 * Possible immunization schedule:
 * Genetic immunization 3 times spaced 3 weeks
 * protein boost does not boost T cell response much
 * Source: Blattmann
 * Genetic immunization day 1, genetic immunization day 3, wait two weeks and then a protein boost
 * CTL responses peak around 6 days after primary immunization or 4 days after boost immunization (Irvine et al., 1997). Spleens or serum can be harvested 2 to 3 weeks after last immunization. (from Current Protocols in Immunology 20.1.19)
 * Mice: B6 mice give better CTL responses than Balb/c mice
 * Source: Blattmann