Start+process+to+construct+transcript+specific+cDNA+for+blunt+end+cloning+and+sequencing

Start process to construct transcript specific cDNA for blunt end cloning and sequencing

associated plan Plan for validating cDNA clones portal 1-7-13

Here are parts of the short names for the primers for the transcripts I would like to amplify

RS61 RW17 RCX3 RRS8 REA RR130 RR130WT RH1 RM1 Rco1

refer to primers Tumor Validation primers 1-28-13

I will just use much less of the 5' oligo to ensure that I have enough to do the reaction (maybe I'll use 1/4).

The PCR products were very smeared on the gel (see 2-13-12 of notebook). I think I may need to use the RACE technique if I want to know the sequences of these transcripts.