Designing+Linkers+071110

A few slides about constructing scFv 7-12-10 "C:\kurt\storage\CIM Research Folder\DR\2013\1-28-13\A few slides about constructing scFv 7-12-10.ppt"

Linker light sequence TTCAGGCGGAGGTGGCTCTGGCGGTGGCGGATCG

Linker heavy sequence CAGAGCCACCTCCGCCTGAACCGCCTCCACCTGAGGAGAC

MHV_BACK_SfiI: CATATGGCCCAGCCGGCCA

MKV_FOR_NOTI: GTTCTTGCGGCCGCCCGT

MLV_FOR_NOTI: GTTCTGCGGCCGCCTTGG

Overlapping Linker Sequence cagagccacctccgcctgaa

Light Chain Overlap with Linker CGATCCGCCACCGCCAGA

Heavy Chain Overlap with Linker GCCTCCACCTGAGGAGAC

Linker amp3 T sequence CGATCCGCCACCGCCAGAGCCACCTCCGCCTGAACCGCCTCCACCTGAGGAGAC

MLV_BACK sequence TTCCTGAGTCACAACAGCCTGCGATCCGCCACCGCCAGA

MHV_FOR1 sequence GCCTCCACCTGCAGAGACAGTGACCAGAGT

original sequence cgatccgccaccgccagagccacctccgcctgaaccgcctccacctgaggagac

Overlapping Linker annealing temp: 57.8 Light chain overlapping temp: 57.0 Heavy chain overlapping temp: 54.7

Overlapping linker primers for scFv Diagram

code for finding temps of all mers for linker 7-13-10 "F:\kurt\storage\CIM Research Folder\DR\2013\6-21-13\wiki_download\code for finding temps of all mers for linker 7-13-10"

Temps of all mers for linker 7-13-10