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e-mail to Kathy

Maybe we should order the mice we need to immunize with ovalbumin.

I'm thinking we could use C57BL/6J mice with the SIINFEKL peptide and B16F10 cells.

The SIINFEKL peptide is a H-2Kb restricted peptide. http://www.sigmaaldrich.com/catalog/product/sigma/s7951?lang=en&region=US

C57BL/6 mice have the H-2Kb haplotype. http://www.imgt.org/IMGTrepertoireMHC/Polymorphism/haplotypes/mouse/MHC/Mu_haplotypes.html#notes

B16F10 cells are derived from C57BL/6J mice. http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=CRL-6475&Template=cellBiology

Maybe we should order 5 of these C57BL/6J mice? Maybe we don't need any for naive controls since non-pulsed cells can act as a control?

Maybe we should wait to order young and old mice until we can show that we can obtain mouse CD34+ cells? I'm thinking I could order anti-mouse CD34+ antibody and purify CD34+ cells from whole blood using FACS.

-Kurt